ArticleBiochimica et biophysica acta. General subjects2017
The underestimated N-glycomes of lepidopteran species.
Article in Biochimica et biophysica acta. General subjects, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Tissue Context Shapes N-Glycan Processing and Site-Specific Microheterogeneity in Adult Drosophila melanogaster Compared With S2 Cells.Archives of insect biochemistry and physiology · 2026Article
- First site-specific N-glycoproteome of the spider mite Tetranychus urticae reveals a glycan signature different from insect models and functional insights for pest management.Experimental & applied acarology · 2026Article
- N-Glycoengineering of insect cells for tri-antennary N-glycan biosynthesis.Scientific reports · 2026Article
- Glycoengineering of nematode antigens using insect cells: a promising approach for producing bioactive vaccine antigens of the barber's pole worm Haemonchus contortus.Glycobiology · 2025Article
- Identification and validation of protective glycoproteins inFrontiers in immunology · 2025Article
- Bioinformatic, Enzymatic, and Structural Characterization ofBiochemistry · 2024Article
- The role of glycoconjugates as receptors for insecticidal proteins.FEMS microbiology reviews · 2023Review
- Increasing Complexity of the N-Glycome During Caenorhabditis Development.Molecular & cellular proteomics : MCP · 2023Article
- Negative-mode mass spectrometry in the analysis of invertebrate, fungal, and protist N-glycans.Mass spectrometry reviews · 2022Review
- Towards understanding the extensive diversity of protein N-glycan structures in eukaryotes.Biological reviews of the Cambridge Philosophical Society · 2022Review
- Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis.Scientific reports · 2021Article
- A bi-specific lectin from the mushroom Boletopsis grisea and its application in glycoanalytical workflows.Scientific reports · 2021Article
- Glycan-dependent cell adhesion mechanism of Tc toxins.Nature communications · 2020Article
- Glycosylation at an evolutionary nexus: the brittle starThe Journal of biological chemistry · 2020Article
- Sulfated and sialylatedThe Journal of biological chemistry · 2020Article
- N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Molecular & cellular proteomics : MCP · 2020Article
- Zwitterionic Phosphodiester-Substituted Neoglycoconjugates as Ligands for Antibodies and Acute Phase Proteins.ACS chemical biology · 2020Article
- Let's talk about sexes: sex-related N-glycosylation in ecologically important invertebrates.Glycoconjugate journal · 2020Review
- Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates.Glycoconjugate journal · 2020Review
- Lectin PLL3, a Novel Monomeric Member of the Seven-Bladed β-Propeller Lectin Family.Molecules (Basel, Switzerland) · 2019Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundInsects are significant to the environment, agriculture, health and biotechnology. Many of these aspects display some relationship to glycosylation, e.g., in case of pathogen binding or production of humanised antibodies; for a long time, it has been considered that insect N-glycosylation potentials are rather similar and simple, but as more species are glycomically analysed in depth, it is becoming obvious that there is indeed a large structural diversity and interspecies variability.
methodsUsing an off-line LC-MALDI-TOF MS approach, we have analysed the N-glycomes of two lepidopteran species (the cabbage looper Trichoplusia ni and the gypsy moth Lymantria dispar) as well as of the commonly-used T. ni High Five cell line.
resultsWe detected not only sulphated, glucuronylated, core difucosylated and Lewis-like antennal fucosylated structures, but also the zwitterion phosphorylcholine on antennal GlcNAc residues, a modification otherwise familiar from nematodes; in L. dispar, N-glycans with glycolipid-like antennae containing α-linked N-acetylgalactosamine were also revealed.
conclusionThe lepidopteran glycomes analysed not only display core α1,3-fucosylation, which is foreign to mammals, but also up to 5% anionic and/or zwitterionic glycans previously not found in these species. SIGNIFICANCE: The occurrence of anionic and zwitterionic glycans in the Lepidoptera data is not only of glycoanalytical and evolutionary interest, but is of biotechnological relevance as lepidopteran cell lines are potential factories for recombinant glycoprotein production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.