ArticleBMC veterinary research2017
Detection of Strongylus vulgaris in equine faecal samples by real-time PCR and larval culture - method comparison and occurrence assessment.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Farm size and biosecurity measures associated with Strongylus vulgaris infection in horses.Equine veterinary journal · 2025Article
- Comparison of morphological and molecular Strongylus spp. identification in equine larval cultures and first report of a patent Strongylus asini infection in a horse.Equine veterinary journal · 2025Article
- Progress in serology and molecular biology of equine parasite diagnosis: sustainable control strategies.Frontiers in veterinary science · 2025Review
- Diagnostic innovations in Equine Parasitology: a Nanogold-ELISA for sensitive serodiagnosis of migratory strongylus vulgaris larvae infections.BMC veterinary research · 2024Article
- A retrospective study of the prevalence in equine postmortems of cranial mesenteric arteritis caused byThe Canadian veterinary journal = La revue veterinaire canadienne · 2024Article
- Selective Anthelmintic Treatment in Horses in Sweden Based on Coprological Analyses: Ten-Year Results.Animals : an open access journal from MDPI · 2023Article
- First microscopic and molecular parasitological survey of Strongylus vulgaris in Brazilian ponies.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2023Article
- Evaluation of Strategies to Reduce Equine Strongyle Infective Larvae on Pasture and Study of Larval Migration and Overwintering in a Nordic Climate.Animals : an open access journal from MDPI · 2022Article
- Morphomolecular characterization ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Occurrence of Strongylid Nematode Parasites on Horse Farms in Berlin and Brandenburg, Germany, With High Seroprevalence ofFrontiers in veterinary science · 2022Article
- Feasibility of selective anthelmintic therapy to horses in tropical conditions: the Cuban scenario.Tropical animal health and production · 2021Article
- Protecting dogs and cats against the Australian paralysis tick,Current research in parasitology & vector-borne diseases · 2021Review
- Intestinal Strongyle Genera in Different Typology of Donkey Farms in Tuscany, Central Italy.Veterinary sciences · 2020Article
- Comparative Analysis of Intestinal Helminth Infections in Colic and Non-Colic Control Equine Patients.Animals : an open access journal from MDPI · 2020Article
- Prevalence ofVeterinary parasitology: X · 2019Article
- Wild horse populations in south-east Australia have a high prevalence ofInternational journal for parasitology. Parasites and wildlife · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStrongylus vulgaris has become a rare parasite in Germany during the past 50 years due to the practice of frequent prophylactic anthelmintic therapy. To date, the emerging development of resistance in Cyathostominae and Parascaris spp. to numerous equine anthelmintics has changed deworming management and the frequency of anthelmintic usage. In this regard, reliable detection of parasitic infections, especially of the highly pathogenic S. vulgaris is essential. In the current study, two diagnostic methods for the detection of infections with S. vulgaris were compared and information on the occurrence of this parasite in German horses was gained. For this purpose, faecal samples of 501 horses were screened for S. vulgaris with real-time PCR and an additional larval culture was performed in samples of 278 horses. A subset of 26 horses underwent multiple follow-up examinations with both methods in order to evaluate both the persistence of S. vulgaris infections and the reproducibility of each diagnostic method.
resultsThe real-time PCR revealed S. vulgaris-DNA in ten of 501 investigated equine samples (1.9%). The larval culture demonstrated larvae of S. vulgaris in three of the 278 samples (1.1%). A direct comparison of the two methods was possible in 321 samples including 43 follow-up examinations with the result of 11 S. vulgaris-positive samples by real-time PCR and 4 S. vulgaris-positive samples by larval culture. The McNemar's test (p-value = 0.016) revealed a significant difference and the kappa values (0.525) showed a moderate agreement between real-time PCR and larval culture.
conclusionsThe real-time PCR detected a significantly higher proportion of positives of S. vulgaris compared to larval culture and should thus be considered as a routine diagnostic method for the detection of S. vulgaris in equine samples.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.