ArticleReproductive biology and endocrinology : RB&E2017
Granulocyte colony-stimulating factor (G-CSF) promotes spermatogenic regeneration from surviving spermatogonia after high-dose alkylating chemotherapy.
Article in Reproductive biology and endocrinology : RB&E, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 32 citations in OpenAlex.
- Single Cell Map of Human Azoospermia Testis Caused by Cyclophosphamide Chemotherapy.Scientific data · 2024Article
- Effect of Granulocyte Colony-Stimulating Factor on the Development of Spermatogenesis in the Adulthood of Juvenile AML Mice Model Treated with Cytarabine.International journal of molecular sciences · 2023Article
- Lovastatin promotes the self-renewal of murine and primate spermatogonial stem cells.Stem cell reports · 2023Article
- Granulocyte-colony stimulating factor does not prevent in vitro cisplatin-induced germ cell reduction in immature human and mouse testis.BMC cancer · 2023Article
- Transcriptional profiling of β-2MStem cell reports · 2022Article
- Granulocyte Colony-Stimulating Factor Restored Impaired Spermatogenesis and Fertility in an AML-Chemotherapy Mice Model.International journal of molecular sciences · 2021Article
- Interaction between DMRT1 and PLZF protein regulates self-renewal and proliferation in male germline stem cells.Molecular and cellular biochemistry · 2021Article
- Knockout of the Transducin-Like Enhancer of Split 6 Gene Affects the Proliferation and Cell Cycle Process of Mouse Spermatogonia.International journal of molecular sciences · 2020Article
- Approaches and Technologies in Male Fertility Preservation.International journal of molecular sciences · 2020Review
- Monophosphoryl lipid A alleviated radiation-induced testicular injury through TLR4-dependent exosomes.Journal of cellular and molecular medicine · 2020Article
- Exposure to Chemotherapy During Childhood or Adulthood and Consequences on Spermatogenesis and Male Fertility.International journal of molecular sciences · 2020Review
- Oncofertility: Pharmacological Protection and Immature Testicular Tissue (ITT)-Based Strategies for Prepubertal and Adolescent Male Cancer Patients.International journal of molecular sciences · 2019Review
- Differential role of r-met-hu G-CSF on male reproductive function and development in prepubertal domestic mammals.PloS one · 2019Article
- Myeloid-derived suppressor cells (MDSCs) in patients with solid tumors: considerations for granulocyte colony-stimulating factor treatment.Cancer immunology, immunotherapy : CII · 2018Review
- Cancer treatment in childhood and testicular function: the importance of the somatic environment.Endocrine connections · 2018Review
- Cytokines in Male Fertility and Reproductive Pathologies: Immunoregulation and Beyond.Frontiers in endocrinology · 2017Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe lifesaving chemotherapy and radiation treatments that allow patients to survive cancer can also result in a lifetime of side-effects, including male infertility. Infertility in male cancer survivors is thought to primarily result from killing of the spermatogonial stem cells (SSCs) responsible for producing spermatozoa since SSCs turn over slowly and are thereby sensitive to antineoplastic therapies. We previously demonstrated that the cytokine granulocyte colony-stimulating factor (G-CSF) can preserve spermatogenesis after alkylating chemotherapy (busulfan).
methodsMale mice were treated with G-CSF or controls before and/or after sterilizing busulfan treatment and evaluated immediately or 10-19 weeks later for effects on spermatogenesis.
resultsWe demonstrated that the protective effect of G-CSF on spermatogenesis was stable for at least 19 weeks after chemotherapy, nearly twice as long as previously shown. Further, G-CSF treatment enhanced spermatogenic measures 10 weeks after treatment in the absence of a cytotoxic insult, suggesting G-CSF acts as a mitogen in steady-state spermatogenesis. In agreement with this conclusion, G-CSF treatment for 3 days before busulfan treatment exacerbated the loss of spermatogenesis observed with G-CSF alone. Reciprocally, spermatogenic recovery was modestly enhanced in mice treated with G-CSF for 4 days after busulfan. These results suggested that G-CSF promoted spermatogonial proliferation, leading to enhanced spermatogenic regeneration from surviving SSCs. Similarly, there was a significant increase in proportion of PLZF+ undifferentiated spermatogonia that were Ki67+ (proliferating) 1 day after G-CSF treatment.
conclusionsTogether, these results clarify that G-CSF protects spermatogenesis after alkylating chemotherapy by stimulating proliferation of surviving spermatogonia, and indicate it may be useful as a retrospective fertility-restoring treatment.
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