Evidence map›Paper›PMID 28076819›Full record

ArticleBiophysical journal2017

Full-Spectral Multiplexing of Bioluminescence Resonance Energy Transfer in Three TRPV Channels.

Hermanus Johannes Ruigrok, Guillaume Shahid, Bertrand Goudeau, Florence Poulletier de Gannes, Emmanuelle Poque-Haro, Annabelle Hurtier, Isabelle Lagroye, Pierre Vacher, Stéphane Arbault, Neso Sojic and 2 more

Open access · bronzeAbstract read
In one paragraph

Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.8field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Genetically-encoded BRET probes shed light on ligand bias-induced variable ion selectivity in TRPV1 and P2X5/7.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  7. Article
  8. Monitoring TRPC7 Conformational Changes by BRET Following GPCR Activation.International journal of molecular sciences · 2022
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 1 country.

Hermanus Johannes RuigrokLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France.
Guillaume ShahidLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France.
Bertrand GoudeauUniversité de Bordeaux, Talence, France; Institut des Sciences Moleculaires, Centre National de la Recherche Scientifique (CNRS) UMR 5255, NSYSA Group, ENSCBP, Pessac, France.
Florence Poulletier de GannesLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France.
Emmanuelle Poque-HaroLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France.
Annabelle HurtierLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France.
Isabelle LagroyeLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France; Paris Sciences et Lettres Research University, Paris, France.
Pierre VacherUniversité de Bordeaux, Talence, France; Institut National de la Santé et de la Recherche Médicale (INSERM) U1218, Institut Bergonié, Bordeaux, France.
Stéphane ArbaultUniversité de Bordeaux, Talence, France; Institut des Sciences Moleculaires, Centre National de la Recherche Scientifique (CNRS) UMR 5255, NSYSA Group, ENSCBP, Pessac, France.
Neso SojicUniversité de Bordeaux, Talence, France; Institut des Sciences Moleculaires, Centre National de la Recherche Scientifique (CNRS) UMR 5255, NSYSA Group, ENSCBP, Pessac, France.
Bernard VeyretLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France.
Yann PercherancierLaboratoire de l'Intégration du Matériau au Système, Centre National de la Recherche Scientifique (CNRS) UMR 5218, Talence, France; Université de Bordeaux, Talence, France. Electronic address: yann.percherancier@ims-bordeaux.fr.
Université de Bordeaux · FRInstitut Polytechnique de Bordeaux · FRCentre National de la Recherche Scientifique · FRÉcole Nationale Supérieure de Chimie, de Biologie et de Physique · FRInserm · FRLaboratoire de l'Intégration du Matériau au Système · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multiplexed bioluminescence resonance energy transfer (BRET) assays were developed to monitor the activation of several functional transient receptor potential (TRP) channels in live cells and in real time. We probed both TRPV1 intramolecular rearrangements and its interaction with Calmodulin (CaM) under activation by chemical agonists and temperature. Our BRET study also confirmed that: (1) capsaicin and heat promoted distinct transitions, independently coupled to channel gating, and that (2) TRPV1 and Ca

Indexed as

Energy TransferLuminescent MeasurementsBiosensing TechniquesCalmodulinHEK293 CellsHot TemperatureHumansTRPV Cation ChannelsCalmodulinTRPV Cation Channels

Identifiers

PMID28076819
PMCPMC5232350
OpenAlexW2569170332

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.