ArticleBiophysical journal2017
3D Protein Dynamics in the Cell Nucleus.
Article in Biophysical journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A predictive mechanochemical modeling framework for the deformation and remodeling of the nuclear lamina.bioRxiv : the preprint server for biology · 2026Article
- Recent advances in nuclear actin research.Nucleus (Austin, Tex.) · 2025Review
- Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy.Communications biology · 2025Article
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- Nanoscale Curvature Regulates YAP/TAZ Nuclear Localization Through Nuclear Deformation and Rupture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Single-molecule imaging for investigating the transcriptional control.Molecules and cells · 2025Review
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Spatially Resolving Size Effects on Diffusivity in Nanoporous Extracellular Matrix-like Materials with Fluorescence Correlation Spectroscopy Super-Resolution Optical Fluctuation Imaging.The journal of physical chemistry. B · 2023Article
- Application of Genetically Encoded Photoconvertible Protein SAASoti for the Study of Enzyme Activity in a Single Live Cell by Fluorescence Correlation Microscopy.Materials (Basel, Switzerland) · 2022Article
- Radial pair correlation of molecular brightness fluctuations maps protein diffusion as a function of oligomeric state within live-cell nuclear architecture.Biophysical journal · 2022Article
- A survey of physical methods for studying nuclear mechanics and mechanobiology.APL bioengineering · 2021Review
- Article
- Single-Particle Tracking Reveals Anti-Persistent Subdiffusion in Cell Extracts.Entropy (Basel, Switzerland) · 2021Article
- Combined Selective Plane Illumination Microscopy and FRAP Maps Intranuclear Diffusion of NLS-GFP.Biophysical journal · 2020Article
- Integrating Microfabrication into Biological Investigations: the Benefits of Interdisciplinarity.Micromachines · 2019Article
- Measuring Mobility in Chromatin by Intensity-Sorted FCS.Biophysical journal · 2019Article
- Nuclear actin: ancient clue to evolution in eukaryotes?Histochemistry and cell biology · 2018Review
- Protein motion in the nucleus: from anomalous diffusion to weak interactions.Biochemical Society transactions · 2018Review
- In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal.Journal of visualized experiments : JoVE · 2018Article
- Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps.Communications biology · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The three-dimensional (3D) architecture of the cell nucleus plays an important role in protein dynamics and in regulating gene expression. However, protein dynamics within the 3D nucleus are poorly understood. Here, we present, to our knowledge, a novel combination of 1) single-objective based light-sheet microscopy, 2) photoconvertible proteins, and 3) fluorescence correlation microscopy, to quantitatively measure 3D protein dynamics in the nucleus. We are able to acquire >3400 autocorrelation functions at multiple spatial positions within a nucleus, without significant photobleaching, allowing us to make reliable estimates of diffusion dynamics. Using this tool, we demonstrate spatial heterogeneity in Polymerase II dynamics in live U2OS cells. Further, we provide detailed measurements of human-Yes-associated protein diffusion dynamics in a human gastric cancer epithelial cell line.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.