ArticlePloS one2017
Effects of Loading Duration and Short Rest Insertion on Cancellous and Cortical Bone Adaptation in the Mouse Tibia.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Methodological aspects ofJournal of musculoskeletal & neuronal interactions · 2023Pooled it
- Combined Effects of Mechanical Loading and Piezo1 Chemical Activation on 22-Months-Old Female Mouse Bone Adaptation.Aging cell · 2025Article
- Differential gene expression in trabecular bone osteocytes is related to the local strain and strain gradient.Scientific reports · 2025Article
- Toward a clear relationship between mechanical signals and bone adaptation.Mechanobiology in medicine · 2025Review
- Intermittent mechanical loading on mouse tibia accelerates longitudinal bone growth by inducing PTHrP expression in the female tibial growth plate.Physiological reports · 2024Article
- Cortical and Trabecular Bone Modeling and Implications for Bone Functional Adaptation in the Mammalian Tibia.Bioengineering (Basel, Switzerland) · 2024Review
- Article
- The loading direction dramatically affects the mechanical properties of the mouse tibia.Frontiers in bioengineering and biotechnology · 2024Article
- Protein Kinase G2 Is Essential for Skeletal Homeostasis and Adaptation to Mechanical Loading in Male but Not Female Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Osteocyte Estrogen Receptor β (Ot-ERβ) Regulates Bone Turnover and Skeletal Adaptive Response to Mechanical Loading Differently in Male and Female Growing and Adult Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Article
- Unraveling the physiologic paradoxes that underlie exercise prescription for stress fracture prevention.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Connexin hemichannels with prostaglandin release in anabolic function of bone to mechanical loading.eLife · 2022Article
- Effects of selective breeding for voluntary exercise, chronic exercise, and their interaction on muscle attachment site morphology in house mice.Journal of anatomy · 2022Article
- Sesamoid bones also show functional adaptation in their microanatomy-The example of the patella in Perissodactyla.Journal of anatomy · 2022Article
- A collagen extraction and deuterium oxide stable isotope tracer method for the quantification of bone collagen synthesis rates in vivo.Physiological reports · 2021Article
- Article
- Application of subject-specific adaptive mechanical loading for bone healing in a mouse tail vertebral defect.Scientific reports · 2021Article
- Cortical bone adaptation to a moderate level of mechanical loading in male Sost deficient mice.Scientific reports · 2020Article
- 3D full-field strain in bone-implant and bone-tooth constructs and their morphological influential factors.Journal of the mechanical behavior of biomedical materials · 2020Article
- Lasting organ-level bone mechanoadaptation is unrelated to local strain.Science advances · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The skeleton's osteogenic response to mechanical loading can be affected by loading duration and rest insertion during a series of loading events. Prior animal loading studies have shown that the cortical bone response saturates quickly and short rest insertions between load cycles can enhance cortical bone formation. However, it remains unknown how loading duration and short rest insertion affect load-induced osteogenesis in the mouse tibial compressive loading model, and particularly in cancellous bone. To address this issue, we applied cyclic loading (-9 N peak load; 4 Hz) to the tibiae of three groups of 16 week-old female C57BL/6 mice for two weeks, with a different number of continuous load cycles applied daily to each group (36, 216 and 1200). A fourth group was loaded under 216 daily load cycles with a 10 s rest insertion after every fourth cycle. We found that as few as 36 load cycles per day were able to induce osteogenic responses in both cancellous and cortical bone. Furthermore, while cortical bone area and thickness continued to increase through 1200 cycles, the incremental increase in the osteogenic response decreased as load number increased, indicating a reduced benefit of the increasing number of load cycles. In the proximal metaphyseal cancellous bone, trabecular thickness increased with load up to 216 cycles. We also found that insertion of a 10 s rest between load cycles did not improve the osteogenic response of the cortical or cancellous tissues compared to continuous loading in this model given the age and sex of the mice and the loading parameters used here. These results suggest that relatively few load cycles (e.g. 36) are sufficient to induce osteogenic responses in both cortical and cancellous bone in the mouse tibial loading model. Mechanistic studies using the mouse tibial loading model to examine bone formation and skeletal mechanobiology could be accomplished with relatively few load cycles.
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