ArticleOncotarget2017
Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype.
Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Propolis-loaded liposomes (ProLip) enhance macrophage-mediated immunomodulation and suppress breast cancer cell proliferation.Scientific reports · 2025Article
- Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness.NPJ breast cancer · 2024Article
- Oncogene activated human breast luminal progenitors contribute basally located myoepithelial cells.Breast cancer research : BCR · 2024Article
- Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness.Research square · 2024Article
- Association of Metabolic and Endocrine Disorders with Bovine Ovarian Follicular Cysts.Animals : an open access journal from MDPI · 2023Article
- Article
- Identification of a core EMT signature that separates basal-like breast cancers into partial- and post-EMT subtypes.Frontiers in oncology · 2023Article
- Myoepithelial progenitors as founder cells of hyperplastic human breast lesions upon PIK3CA transformation.Communications biology · 2022Article
- Desensitization of human breast progenitors by a transient exposure to pregnancy levels of estrogen.Scientific reports · 2021Article
- Fibroblasts direct differentiation of human breast epithelial progenitors.Breast cancer research : BCR · 2020Article
- Modeling chemical effects on breast cancer: the importance of the microenvironment in vitro.Integrative biology : quantitative biosciences from nano to macro · 2020Review
- A CD146 FACS Protocol Enriches for Luminal Keratin 14/19 Double Positive Human Breast Progenitors.Scientific reports · 2019Article
- Mammary adipose stromal cells derived from obese women reduce sensitivity to the aromatase inhibitor anastrazole in an organotypic breast model.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Application of the D492 Cell Lines to Explore Breast Morphogenesis, EMT and Cancer Progression in 3D Culture.Journal of mammary gland biology and neoplasia · 2019Review
- Normal Breast-Derived Epithelial Cells with Luminal and Intrinsic Subtype-Enriched Gene Expression Document Interindividual Differences in Their Differentiation Cascade.Cancer research · 2018Article
- Proliferative heterogeneity of murine epithelial cells in the adult mammary gland.Communications biology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding human cancer increasingly relies on insight gained from subtype specific comparisons between malignant and non-malignant cells. The most frequent subtype in breast cancer is the luminal. By far the most frequently used model for luminal breast cancer is the iconic estrogen receptor-positive (ERpos) MCF7 cell line. However, luminal specific comparisons have suffered from the lack of a relevant non-malignant counterpart. Our previous work has shown that transforming growth factor-β receptor (TGFβR) inhibition suffices to propagate prospectively isolated ERpos human breast luminal cells from reduction mammoplasties (HBEC). Here we demonstrate that transduction of these cells with hTERT/shp16 renders them immortal while remaining true to the luminal lineage including expression of functional ER (iHBECERpos). Under identical culture conditions a major difference between MCF7 and normal-derived cells is the dependence of the latter on TGFβR inhibition for ER expression. In a breast fibroblast co-culture model we further show that whereas MCF7 proliferate concurrently with ER expression, iHBECERpos form correctly polarized acini, and segregate into proliferating and ER expressing cells. We propose that iHBECERpos may serve to shed light on hitherto unappreciated differences in ER regulation and function between normal breast and breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.