ArticleScientific reports2017
Multi-cellular natural killer (NK) cell clusters enhance NK cell activation through localizing IL-2 within the cluster.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Feeder-free ex vivo expansion of cord blood-derived natural killer cells for enhanced proliferation and functional maturation.Scientific reports · 2026Article
- Analysis of Phenotypic and Molecular Variability of Memory-like NK Cells for Cancer Adoptive Cell Therapy Screening.Cancers · 2025Article
- Connexin 43 Production by Ex Vivo Incubated NK Cells in the Presence of Relevant Cytokine Combinations.Bulletin of experimental biology and medicine · 2025Article
- CD301bNature communications · 2025Article
- Biomaterial-Based Therapeutic Delivery of Immune Cells.Advanced healthcare materials · 2025Review
- NK cells in HPV-related tumorigenesis: mechanisms and clinical applications.Frontiers in cellular and infection microbiology · 2025Review
- High-throughput proliferation and activation of NK-92MI cell spheroids via a homemade one-step closed bioreactor in pseudostatic cultures for immunocellular therapy.Journal of biological engineering · 2024Article
- Networked Cluster Formation via Trigonal Lipid Modules for Augmented Ex Vivo NK Cell Priming.International journal of molecular sciences · 2024Article
- CD4 Effector TCR Avidity for Peptide on APC Determines the Level of Memory Generated.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Cytokine engineered NK-92 therapy to improve persistence and anti-tumor activity.Theranostics · 2023Article
- Analysis of the impact of handling and culture on the expansion and functionality of NK cells.Frontiers in immunology · 2023Article
- Biomaterials for enhanced immunotherapy.APL bioengineering · 2022Review
- Metabolic diversity within breast cancer brain-tropic cells determines metastatic fitness.Cell metabolism · 2022Article
- Effects of extracellular adhesion molecules on immune cell mediated solid tumor cell killing.Frontiers in immunology · 2022Article
- AHR Regulates NK Cell MigrationFrontiers in immunology · 2021Article
- Towards systems tissue engineering: Elucidating the dynamics, spatial coordination, and individual cells driving emergent behaviors.Biomaterials · 2020Review
- Extracellular NK histones promote immune cell anti-tumor activity by inducing cell clusters through binding to CD138 receptor.Journal for immunotherapy of cancer · 2019Article
- Gene Therapy with an Adeno-Associated Viral Vector Expressing Human Interleukin-2 Alters Immune System Homeostasis in Humanized Mice.Human gene therapy · 2018Article
- The Molecular Mechanism of Natural Killer Cells Function and Its Importance in Cancer Immunotherapy.Frontiers in immunology · 2017Review
- Memory of Natural Killer Cells: A New Chance againstFrontiers in immunology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multi-cellular cluster formation of natural killer (NK) cells occurs during in vivo priming and potentiates their activation to IL-2. However, the precise mechanism underlying this synergy within NK cell clusters remains unclear. We employed lymphocyte-laden microwell technologies to modulate contact-mediated multi-cellular interactions among activating NK cells and to quantitatively assess the molecular events occurring in multi-cellular clusters of NK cells. NK cells in social microwells, which allow cell-to-cell contact, exhibited significantly higher levels of IL-2 receptor (IL-2R) signaling compared with those in lonesome microwells, which prevent intercellular contact. Further, CD25, an IL-2R α chain, and lytic granules of NK cells in social microwells were polarized toward MTOC. Live cell imaging of lytic granules revealed their dynamic and prolonged polarization toward neighboring NK cells without degranulation. These results suggest that IL-2 bound on CD25 of one NK cells triggered IL-2 signaling of neighboring NK cells. These results were further corroborated by findings that CD25-KO NK cells exhibited lower proliferation than WT NK cells, and when mixed with WT NK cells, underwent significantly higher level of proliferation. These data highlights the existence of IL-2 trans-presentation between NK cells in the local microenvironment where the availability of IL-2 is limited.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.