ArticleScientific reports2017
Two-Layer Elastographic 3-D Traction Force Microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The extracellular matrix mechanics in the vasculature.Nature cardiovascular research · 2023Review
- Quantifying stiffness and forces of tumor colonies and embryos using a magnetic microrobot.Science robotics · 2023Article
- Biomechanical interactions of Schistosoma mansoni eggs with vascular endothelial cells facilitate egg extravasation.PLoS pathogens · 2022Article
- A Stiff Extracellular Matrix Favors the Mechanical Cell Competition that Leads to Extrusion of Bacterially-Infected Epithelial Cells.Frontiers in cell and developmental biology · 2022Article
- Quantifying cell-generated forces: Poisson's ratio matters.Communications physics · 2021Article
- Cytoskeletal prestress: The cellular hallmark in mechanobiology and mechanomedicine.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Elucidating the Biomechanics of Leukocyte Transendothelial Migration by Quantitative Imaging.Frontiers in cell and developmental biology · 2021Review
- Quantifying cellular forces: Practical considerations of traction force microscopy for dermal fibroblasts.Experimental dermatology · 2021Review
- Measuring mechanical stress in living tissues.Nature reviews. Physics · 2020Article
- Design, Implementation, and Validation of a Piezoelectric Device to Study the Effects of Dynamic Mechanical Stimulation on Cell Proliferation, Migration and Morphology.Sensors (Basel, Switzerland) · 2020Article
- Matrigel patterning reflects multicellular contractility.PLoS computational biology · 2019Article
- Three-Dimensional Monolayer Stress Microscopy.Biophysical journal · 2019Article
- Quantifying the mechanics of locomotion of the schistosome pathogen with respect to changes in its physical environment.Journal of the Royal Society, Interface · 2019Article
- Holographic Traction Force Microscopy.Scientific reports · 2018Article
- Self-organized mechano-chemical dynamics in amoeboid locomotion ofJournal of physics D: Applied physics · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Cellular traction force microscopy (TFM) requires knowledge of the mechanical properties of the substratum where the cells adhere to calculate cell-generated forces from measurements of substratum deformation. Polymer-based hydrogels are broadly used for TFM due to their linearly elastic behavior in the range of measured deformations. However, the calculated stresses, particularly their spatial patterns, can be highly sensitive to the substratum's Poisson's ratio. We present two-layer elastographic TFM (2LETFM), a method that allows for simultaneously measuring the Poisson's ratio of the substratum while also determining the cell-generated forces. The new method exploits the analytical solution of the elastostatic equation and deformation measurements from two layers of the substratum. We perform an in silico analysis of 2LETFM concluding that this technique is robust with respect to TFM experimental parameters, and remains accurate even for noisy measurement data. We also provide experimental proof of principle of 2LETFM by simultaneously measuring the stresses exerted by migrating Physarum amoeboae on the surface of polyacrylamide substrata, and the Poisson's ratio of the substrata. The 2LETFM method could be generalized to concurrently determine the mechanical properties and cell-generated forces in more physiologically relevant extracellular environments, opening new possibilities to study cell-matrix interactions.
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