ArticlePlanta2017
NaCl stress induces CsSAMs gene expression in Cucumis sativus by mediating the binding of CsGT-3b to the GT-1 element within the CsSAMs promoter.
Article in Planta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 35 citations in OpenAlex.
- Genome-wide identification and functional analysis of the SAMS gene family in peanut reveal the role of Ah6Q1KS5 in resistance to bacterial wilt.BMC plant biology · 2026Article
- Genome-wide identification of the GT transcription factor family in alfalfa and functional characterization of MsGT16 in salt stress tolerance.BMC genomics · 2026Article
- Genome-Wide Identification and Characterization of the Trihelix Transcription Factor Family inPlants (Basel, Switzerland) · 2025Article
- Genome-wide identification and expression analysis of trihelix transcription factor family in cucumber (Cucumis sativus L.) and their roles in biotic stress responses.BMC genomics · 2025Article
- Comprehensive Genomic Analysis of Trihelix Transcription Factor Genes and Their Expression Underlying Abiotic Stress in Euphrates Poplar (Plants (Basel, Switzerland) · 2025Article
- Article
- Fine mapping and identification of ERF transcription factor ERF017 as a candidate gene for cold tolerance in pumpkin.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Artificially designed synthetic promoter for a high level of salt induction using a cis-engineering approach.Scientific reports · 2024Article
- Molecular Mechanism of Cold Tolerance of Centipedegrass Based on the Transcriptome.International journal of molecular sciences · 2023Article
- Genome-wide characterization and identification of Trihelix transcription factors and expression profiling in response to abiotic stresses in Chinese Willow (Frontiers in plant science · 2023Article
- Identification of cucumber S-adenosylmethionine decarboxylase genes and functional analysis ofFrontiers in plant science · 2023Article
- Trihelix Transcriptional Factor GhGT26 of Cotton Enhances Salinity Tolerance inPlants (Basel, Switzerland) · 2022Article
- MsSAMS, a cold stress-responsive gene, provides resistance to environmental stress in TTransgenic research · 2022Article
- Article
- Isolation and functional validation of the CmLOX08 promoter associated with signalling molecule and abiotic stress responses in oriental melon, Cucumis melo var. makuwa Makino.BMC plant biology · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionThe CsSAMs promoter is a salt-stress-inducible promoter containing three GT-1 elements that are sufficient for the salt-stress response. The transcription factor CsGT-3b was found to bind to the GT-1 element. The S-adenosyl-L-methionine synthase (SAMs) gene is among the functional genes induced during environmental stress. However, little is known about the regulatory mechanism and upstream regulators of this salt-inducible gene in cucumber plants. Thus, it is necessary to understand the characteristics of the SAMs gene by analyzing its promoter and transcription factors. In this study, we isolated and functionally analyzed a 1743-bp flanking fragment of the CsSAMs gene from Cucumis sativus. To examine promoter activity, the full-length promoter, as well as different promoter fragments, were fused to the β-glucuronidase (GUS) reporter gene and introduced into the tobacco genome. The full-length promoter displayed maximal promoter activity, whereas the P4 promoter, containing 321 bp of upstream sequence, showed no basal promoter activity. In addition, the CsSAMs promoter exhibited stress-inducible regulation rather than tissue-specific activity in transgenic tobacco. Histochemical analysis revealed strong GUS staining in leaves, stems, and roots, especially in the veins of leaves, the vascular bundle of stems, and root tip zones following NaCl stress. A transient expression assay confirmed that the 242-bp region (-1743 to -1500) was sufficient for the NaCl-stress response. Yeast one-hybrid assays further revealed interaction between the NaCl-response protein CsGT-3b and the GT-1 (GAAAAA) element within the 242-bp region. Taken together, we revealed the presence of four salt-stress-responsive elements (GT-1 cis-elements) in the CsSAMs promoter and identified a transcription factor, CsGT-3b, that specifically binds to this sequence. These results might help us better understand the intricate regulatory network of the cucumber SAMs gene.
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