ArticleJournal of biological engineering2017
Cellulolytic RoboLector - towards an automated high-throughput screening platform for recombinant cellulase expression.
Article in Journal of biological engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 64 citations in OpenAlex.
- From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles.Microbial cell factories · 2023Article
- Assessing the capabilities of 2D fluorescence monitoring in microtiter plates with data-driven modeling for secondary substrate limitation experiments of Hansenula polymorpha.Journal of biological engineering · 2023Article
- Patterning of the Autotrophic, Mixotrophic, and Heterotrophic Proteomes of Oxygen-Evolving CyanobacteriumFrontiers in microbiology · 2022Article
- Fungal cellulases: protein engineering and post-translational modifications.Applied microbiology and biotechnology · 2022Review
- Robotics for enzyme technology: innovations and technological perspectives.Applied microbiology and biotechnology · 2021Review
- Screening for optimal protease producing Bacillus licheniformis strains with polymer-based controlled-release fed-batch microtiter plates.Microbial cell factories · 2021Article
- Ethanol tolerance assessment in recombinant E. coli of ethanol responsive genes from Lactobacillus buchneri NRRL B-30929.World journal of microbiology & biotechnology · 2020Article
- Parallelized disruption of prokaryotic and eukaryotic cells via miniaturized and automated bead mill.Engineering in life sciences · 2020Article
- Disulfide Bond Engineering of an Endoglucanase fromInternational journal of molecular sciences · 2019Article
- Polymer-based controlled-release fed-batch microtiter plate - diminishing the gap between early process development and production conditions.Journal of biological engineering · 2019Article
- Online evaluation of the metabolic activity ofJournal of biological engineering · 2018Article
- Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures.Microbial cell factories · 2017Article
- Prediction ofJournal of biological engineering · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings.
resultsIn this study, an extended robotic platform is presented to conduct automated high-throughput screenings of clone libraries including preculture synchronization and biomass specific induction. Automated upstream, downstream and analytical process steps are described and evaluated using
conclusionThe RoboLector device is a suitable platform to perform all process steps of an automated high-throughput clone library screening for improved cellulases. On-line biomass growth measurement controlling liquid handling actions enables fair comparison of clone variants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.