ArticleProceedings of the National Academy of Sciences of the United States of America2017
Redox crisis underlies conditional light-dark lethality in cyanobacterial mutants that lack the circadian regulator, RpaA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 66 citations in OpenAlex.
- Thiol post-translational modifications modulate allosteric regulation of the OpcA-G6PDH complex through conformational gate control.Protein science : a publication of the Protein Society · 2026Article
- Aging of Microplastics and Nanoplastics Induced by Advanced Oxidation Processes in Wastewater Treatment and Their Biological Toxicity: A Systematic Review.Microorganisms · 2026Review
- Deciphering the metabolic details of L-lysine toxicity in cyanobacteria.Plant physiology · 2026Article
- From photosynthetic electron flow to gene regulation: redox signal transduction in cyanobacteria.FEMS microbiology reviews · 2026Review
- Optimising environmental factors for maximal lactate productivity in Synechocystis sp. PCC 6803 through a design of experiments approach.Biotechnology for biofuels and bioproducts · 2025Article
- Diurnal rhythm causes metabolic crises in the cyanobacterial mutants of c-di-AMP signaling cascade.iScience · 2025Article
- Mutations in the circadian cycle drive adaptive plasticity in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Plastoquinone redox status influences carboxysome integrity via a RpaA- and reactive oxygen species-dependent regulatory network.The Plant journal : for cell and molecular biology · 2025Article
- Impacts of PAR and UV radiation on diurnal photosynthesis performance, pigment composition, and antioxidant function of the hot-spring cyanobacterium Nostoc sp. strain VKB02.Archives of microbiology · 2025Article
- Phosphoketolase and KDPG aldolase metabolisms modulate photosynthetic carbon yield in cyanobacteria.The Plant cell · 2024Article
- Universal Lineage-Independent Markers of Multidrug Resistance inMicroorganisms · 2024Article
- Article
- Emergence of metabolic coupling to the heterotrophISME communications · 2024Article
- Circadian regulation of metabolism across photosynthetic organisms.The Plant journal : for cell and molecular biology · 2023Review
- Glycogen metabolism is required for optimal cyanobacterial growth in the rapid light-dark cycle of low-Earth orbit.Life sciences in space research · 2023Article
- To Die or Not to Die-Regulated Cell Death and Survival in Cyanobacteria.Microorganisms · 2022Review
- Comparative Genomics of Synechococcus elongatus Explains the Phenotypic Diversity of the Strains.mBio · 2022Article
- Thiol redox switches regulate the oligomeric state of cyanobacterial Rre1, RpaA and RpaB response regulators.FEBS letters · 2022Article
- A Global Metabolic Map Defines the Effects of a Si-Based Biostimulant on Tomato Plants under Normal and Saline Conditions.Metabolites · 2021Article
- Shift in Conformational Equilibrium Underlies the Oscillatory Phosphoryl Transfer Reaction in the Circadian Clock.Life (Basel, Switzerland) · 2021Article
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6 authors at 1 institution in 1 country.
Funding
Abstract
Cyanobacteria evolved a robust circadian clock, which has a profound influence on fitness and metabolism under daily light-dark (LD) cycles. In the model cyanobacterium Synechococcus elongatus PCC 7942, a functional clock is not required for diurnal growth, but mutants defective for the response regulator that mediates transcriptional rhythms in the wild-type, regulator of phycobilisome association A (RpaA), cannot be cultured under LD conditions. We found that rpaA-null mutants are inviable after several hours in the dark and compared the metabolomes of wild-type and rpaA-null strains to identify the source of lethality. Here, we show that the wild-type metabolome is very stable throughout the night, and this stability is lost in the absence of RpaA. Additionally, an rpaA mutant accumulates excessive reactive oxygen species (ROS) during the day and is unable to clear it during the night. The rpaA-null metabolome indicates that these cells are reductant-starved in the dark, likely because enzymes of the primary nighttime NADPH-producing pathway are direct targets of RpaA. Because NADPH is required for processes that detoxify ROS, conditional LD lethality likely results from inability of the mutant to activate reductant-requiring pathways that detoxify ROS when photosynthesis is not active. We identified second-site mutations and growth conditions that suppress LD lethality in the mutant background that support these conclusions. These results provide a mechanistic explanation as to why rpaA-null mutants die in the dark, further connect the clock to metabolism under diurnal growth, and indicate that RpaA likely has important unidentified functions during the day.
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