ArticleFEMS yeast research2017
Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase.
Article in FEMS yeast research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Comprehensive review of biosynthesis, plant function, metabolism and new therapeutic bioactivities of flavonoids.ADMET & DMPK · 2026Review
- Reinforced NADPH regeneration in engineered Saccharomyces cerevisiae enhances rosmarinic acid production.Archives of microbiology · 2025Article
- Systems Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2024Article
- High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis inACS synthetic biology · 2023Article
- Raspberry Ketone Accumulation inJournal of agricultural and food chemistry · 2023Article
- Optimization of Pinocembrin Biosynthesis inACS synthetic biology · 2023Article
- AACS synthetic biology · 2022Article
- Metabolic engineering of Yarrowia lipolytica for scutellarin production.Synthetic and systems biotechnology · 2022Article
- A whiff of the future: functions of phenylalanine-derived aroma compounds and advances in their industrial production.Plant biotechnology journal · 2022Review
- MULTI-SCULPT: Multiplex Integration via Selective, CRISPR-Mediated, Ultralong Pathway Transformation in Yeast for Plant Natural Product Synthesis.ACS synthetic biology · 2022Article
- Article
- Synthetic Biology towards Improved Flavonoid Pharmacokinetics.Biomolecules · 2021Review
- Treasure from garden: Bioactive compounds of buckwheat.Food chemistry · 2021Article
- 4-Coumaroyl-CoA ligases in the biosynthesis of the anti-diabetic metabolite montbretin A.PloS one · 2021Article
- Membrane transporters: the key drivers of transport of secondary metabolites in plants.Plant cell reports · 2021Review
- Recent Advances in Heterologous Synthesis Paving Way for Future Green-Modular Bioindustries: A Review With Special Reference to Isoflavonoids.Frontiers in bioengineering and biotechnology · 2021Review
- Yeasts as Biopharmaceutical Production Platforms.Frontiers in fungal biology · 2021Article
- Discovery of a previously unknown biosynthetic capacity of naringenin chalcone synthase by heterologous expression of a tomato gene cluster in yeast.Science advances · 2020Article
- Toward Developing a Yeast Cell Factory for the Production of Prenylated Flavonoids.Journal of agricultural and food chemistry · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenylpropanoids, such as flavonoids and stilbenoids, are of great commercial interest, and their production in Saccharomyces cerevisiae is a very promising strategy. However, to achieve commercially viable production, each step of the process must be optimised. We looked at carbon loss, known to occur in the heterologous flavonoid pathway in yeast, and identified an endogenous enzyme, the enoyl reductase Tsc13, which turned out to be responsible for the accumulation of phloretic acid via reduction of p-coumaroyl-CoA. Tsc13 is an essential enzyme involved in fatty acid synthesis and cannot be deleted. Hence, two approaches were adopted in an attempt to reduce the side activity without disrupting the natural function: site saturation mutagenesis identified a number of amino acid changes which slightly increased flavonoid production but without reducing the formation of the side product. Conversely, the complementation of TSC13 by a plant gene homologue essentially eliminated the unwanted side reaction, while retaining the productivity of phenylpropanoids in a simulated fed batch fermentation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.