ArticleNano letters2017
Fluorescence Self-Quenching from Reporter Dyes Informs on the Structural Properties of Amyloid Clusters Formed in Vitro and in Cells.
Article in Nano letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 87 citations in OpenAlex.
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- Discovery of TDP-43 aggregation inhibitorsbioRxiv : the preprint server for biology · 2026Article
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- Deep Learning for Fluorescence Lifetime Predictions Enables High-Throughput In Vivo Imaging.Journal of the American Chemical Society · 2025Article
- Mechanisms of Alpha-Synuclein-Seeded Aggregation in Neurons Revealed by Fluorescence Lifetime Imaging.ACS chemical neuroscience · 2025Article
- Modulation of insulin aggregation by betaine and proline directly observed via real-time super-resolution microscopy.Protein science : a publication of the Protein Society · 2025Article
- The role of RNA in the nanoscale organization of α-synuclein phase separation.NAR molecular medicine · 2025Article
- Collagen-targeted protein nanomicelles for the imaging of non-alcoholic steatohepatitis.Acta biomaterialia · 2024Article
- Extended voltage imaging in cardiomyocytes with a triplet state quencher-stabilized silicon rhodamine.Bioorganic & medicinal chemistry letters · 2024Article
- Unveiling Morphine: A Rapid and Selective Fluorescence Sensor for Forensic and Medical Analysis.Sensors (Basel, Switzerland) · 2024Article
- Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024Review
- BaGlobal challenges (Hoboken, NJ) · 2024Article
- Uptake Quantification of Antigen Carried by Nanoparticles and Its Impact on Carrier Adjuvanticity Evaluation.Vaccines · 2023Article
- Synthesis of Fluorescent Cyclic Peptides via Gold(I)-Catalyzed Macrocyclization.Journal of the American Chemical Society · 2023Article
- Probing cell membrane integrity using a histone-targeting protein nanocage displaying precisely positioned fluorophores.Nano research · 2023Article
- Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM.Communications biology · 2022Article
- Direct observation of heterogeneous formation of amyloid spherulites in real-time by super-resolution microscopy.Communications biology · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The characterization of the aggregation kinetics of protein amyloids and the structural properties of the ensuing aggregates are vital in the study of the pathogenesis of many neurodegenerative diseases and the discovery of therapeutic targets. In this article, we show that the fluorescence lifetime of synthetic dyes covalently attached to amyloid proteins informs on the structural properties of amyloid clusters formed both in vitro and in cells. We demonstrate that the mechanism behind such a "lifetime sensor" of protein aggregation is based on fluorescence self-quenching and that it offers a good dynamic range to report on various stages of aggregation without significantly perturbing the process under investigation. We show that the sensor informs on the structural density of amyloid clusters in a high-throughput and quantitative manner and in these aspects the sensor outperforms super-resolution imaging techniques. We demonstrate the power and speed of the method, offering capabilities, for example, in therapeutic screenings that monitor biological self-assembly. We investigate the mechanism and advantages of the lifetime sensor in studies of the K18 protein fragment of the Alzheimer's disease related protein tau and its amyloid aggregates formed in vitro. Finally, we demonstrate the sensor in the study of aggregates of polyglutamine protein, a model used in studies related to Huntington's disease, by performing correlative fluorescence lifetime imaging microscopy and structured-illumination microscopy experiments in cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.