ArticleBiosensors & bioelectronics2017
Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus.
Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 106 citations in OpenAlex.
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- Comparison of Illumination Methods for Flow-Through Optofluidic Biosensors.Micromachines · 2023Article
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- Toward smart diagnosis of pandemic infectious diseases using wastewater-based epidemiology.Trends in analytical chemistry : TRAC · 2022Review
- Smartphone-Based Multiplexed Biosensing Tools for Health Monitoring.Biosensors · 2022Review
- Virus Detection: From State-of-the-Art Laboratories to Smartphone-Based Point-of-Care Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis.IEEE photonics journal · 2022Article
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- Advances in multiplex electrical and optical detection of biomarkers using microfluidic devices.Analytical and bioanalytical chemistry · 2022Review
- Micro and Nanoscale Technologies for Diagnosis of Viral Infections.Small (Weinheim an der Bergstrasse, Germany) · 2021Review
- Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics.Biosensors · 2021Review
- Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Article
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Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
An automated microfluidic sample preparation multiplexer (SPM) has been developed and evaluated for Ebola virus detection. Metered air bubbles controlled by microvalves are used to improve bead-solution mixing thereby enhancing the hybridization of the target Ebola virus RNA with capture probes bound to the beads. The method uses thermally stable 4-formyl benzamide functionalized (4FB) magnetic beads rather than streptavidin coated beads with a high density of capture probes to improve the target capture efficiency. Exploiting an on-chip concentration protocol in the SPM and the single molecule detection capability of the antiresonant reflecting optical waveguide (ARROW) biosensor chip, a detection limit of 0.021pfu/mL for clinical samples is achieved without target amplification. This RNA target capture efficiency is two orders of magnitude higher than previous results using streptavidin beads and the limit of detection (LOD) improves 10×. The wide dynamic range of this technique covers the whole clinically applicable concentration range. In addition, the current sample preparation time is ~1h which is eight times faster than previous work. This multiplexed, miniaturized sample preparation microdevice establishes a key technology that intended to develop next generation point-of-care (POC) detection system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.