ArticleDevelopmental cell2017
CENP-A Modifications on Ser68 and Lys124 Are Dispensable for Establishment, Maintenance, and Long-Term Function of Human Centromeres.
Article in Developmental cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Article
- Centromeric chromatin clearings demarcate the site of kinetochore formation.bioRxiv : the preprint server for biology · 2024Article
- The Roles of Histone Post-Translational Modifications in the Formation and Function of a Mitotic Chromosome.International journal of molecular sciences · 2022Review
- Non-proteolytic ubiquitylation in cellular signaling and human disease.Communications biology · 2022Review
- Gene replacement strategies validate the use of functional tags on centromeric chromatin and invalidate an essential role for CENP-ACell reports · 2021Article
- RbAp46/48LIN-53 and HAT-1 are required for initial CENP-AHCP-3 deposition and de novo holocentromere formation on artificial chromosomes in Caenorhabditis elegans embryos.Nucleic acids research · 2021Article
- Conservation of centromeric histone 3 interaction partners in plants.Journal of experimental botany · 2020Article
- Epigenetic regulation of centromere function.Cellular and molecular life sciences : CMLS · 2020Review
- Review
- Article
- Priming chromatin for segregation: functional roles of mitotic histone modifications.Cell cycle (Georgetown, Tex.) · 2020Review
- Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading.Nucleic acids research · 2019Article
- CENP-A Ubiquitylation Is Indispensable to Cell Viability.Developmental cell · 2019Article
- TAR Cloning: Perspectives for Functional Genomics, Biomedicine, and Biotechnology.Molecular therapy. Methods & clinical development · 2019Review
- Article
- CDK phosphorylation ofThe EMBO journal · 2019Article
- The CENP-A centromere targeting domain facilitates H4K20 monomethylation in the nucleosome by structural polymorphism.Nature communications · 2019Article
- Phosphorylation of CENP-A on serine 7 does not control centromere function.Nature communications · 2019Article
- The dark side of centromeres: types, causes and consequences of structural abnormalities implicating centromeric DNA.Nature communications · 2018Review
- Posttranslational modifications of CENP-A: marks of distinction.Chromosoma · 2018Review
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Abstract
CENP-A is a histone H3 variant key to epigenetic specification of mammalian centromeres. Using transient overexpression of CENP-A mutants, two recent reports in Developmental Cell proposed essential centromere functions for post-translational modifications of human CENP-A. Phosphorylation at Ser68 was proposed to have an essential role in CENP-A deposition at centromeres. Blockage of ubiquitination at Lys124 was proposed to abrogate localization of CENP-A to the centromere. Following gene inactivation and replacement in human cells, we demonstrate that CENP-A mutants that cannot be phosphorylated at Ser68 or ubiquitinated at Lys124 assemble efficiently at centromeres during G1, mediate early events in centromere establishment at an ectopic chromosomal locus, and maintain centromere function indefinitely. Thus, neither Ser68 nor Lys124 post-translational modification is essential for long-term centromere identity, propagation, cell-cycle-dependent deposition, maintenance, function, or mediation of early steps in centromere establishment.
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