ArticleScientific reports2017
Generation of germline ablated male pigs by CRISPR/Cas9 editing of the NANOS2 gene.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 135 citations in OpenAlex.
- Horizon scanning of potential environmental applications of terrestrial animals, fish, algae and microorganisms produced by genetic modification, including the use of new genomic techniques.Frontiers in genome editing · 2024Pooled it
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Functional Inactivation ofInternational journal of molecular sciences · 2026Article
- Male germ cell technologies in large animals.The Journal of reproduction and development · 2026Review
- Insulin-like growth factor 1 receptor (Frontiers in cell and developmental biology · 2026Article
- Considerations for recipient male selection in a livestock Surrogate Sire breeding strategy.The Journal of reproduction and development · 2026Review
- Pig Genome Editing for Agriculture: Achievements and Challenges.International journal of molecular sciences · 2025Review
- Serum norethisterone (NET) levels in NET-enanthate (NET-EN) injectable contraception users substantially interfere with testosterone immunoassay measurements and confound interpretation of biological outcomes.Contraception and reproductive medicine · 2025Article
- Morula complementation restores male germline inPNAS nexus · 2025Article
- Hormonal contraceptives during adolescence impact the female brain and behavior in a rat model.Hormones and behavior · 2025Article
- A bibliometric mapping of advancements and trends in genome editing in pigs.Tropical animal health and production · 2025Review
- Comparison of assay methods for quantifying sex hormone concentrations across the menstrual cycle in rhesus macaques†.Biology of reproduction · 2024Article
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- Livestock embryonic stem cells for reproductive biotechniques and genetic improvement.Animal reproduction · 2024Article
- Article
- The history, use, and challenges of therapeutic somatic cell and germline gene editing.Fertility and sterility · 2023Review
- Inactivation of growth differentiation factor 9 blocks folliculogenesis in pigs†.Biology of reproduction · 2023Article
- Germline ablation achieved via CRISPR/Cas9 targeting ofFrontiers in genome editing · 2023Article
- Generation of germ cell-deficient pigs by NANOS3 knockout.The Journal of reproduction and development · 2022Article
- Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
Abstract
Genome editing tools have revolutionized the generation of genetically modified animals including livestock. In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR/Cas9 system to edit the NANOS2 gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that NANOS2 knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact NANOS2 allele and female knockout pigs are fertile. From an agriculture perspective, NANOS2 knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.