Evidence map›Paper›PMID 28071667›Full record

ArticleNature communications2017

PhotoGate microscopy to track single molecules in crowded environments.

Vladislav Belyy, Sheng-Min Shih, Jigar Bandaria, Yongjian Huang, Rosalie E Lawrence, Roberto Zoncu, Ahmet Yildiz

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Single-cell analysis targeting the proteome.Nature reviews. Chemistry · 2020
    Review
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Vladislav BelyyBiophysics Graduate Group, University of California, Berkeley, California 94720, USA.
Sheng-Min ShihDepartment of Physics, University of California, Berkeley, California 94720, USA.
Jigar BandariaDepartment of Physics, University of California, Berkeley, California 94720, USA.
Yongjian HuangBiophysics Graduate Group, University of California, Berkeley, California 94720, USA.
Rosalie E LawrenceDepartment of Molecular Cell Biology, University of California, Berkeley, California 94720, USA.
Roberto ZoncuDepartment of Molecular Cell Biology, University of California, Berkeley, California 94720, USA.
Ahmet YildizBiophysics Graduate Group, University of California, Berkeley, California 94720, USA.
University of California, Berkeley · US

Funding

Understanding the Mechanism and Regulation of the Human Cytoplasmic Dynein ComplexR01GM094522 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI YILDIZ, AHMET · 2011 to 2019
$2.8M
Structural and Functional Characterization of Telomere Protection and MaintenanceR01GM118773 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI YILDIZ, AHMET · 2016 to 2019
$1.2M
NIGMS NIH HHS R01 GM094522NIGMS NIH HHS R01 GM118773
6 · The paper itself

Abstract

Tracking single molecules inside cells reveals the dynamics of biological processes, including receptor trafficking, signalling and cargo transport. However, individual molecules often cannot be resolved inside cells due to their high density. Here we develop the PhotoGate technique that controls the number of fluorescent particles in a region of interest by repeatedly photobleaching its boundary. PhotoGate bypasses the requirement of photoactivation to track single particles at surface densities two orders of magnitude greater than the single-molecule detection limit. Using this method, we observe ligand-induced dimerization of a receptor tyrosine kinase at the cell surface and directly measure binding and dissociation of signalling molecules from early endosomes in a dense cytoplasm with single-molecule resolution. We additionally develop a numerical simulation suite for rapid quantitative optimization of Photogate experimental conditions. PhotoGate yields longer tracking times and more accurate measurements of complex stoichiometry than existing single-molecule imaging methods.

Indexed as

Adaptor Proteins, Signal TransducingAnimalsCell MembraneChlorocebus aethiopsComputer SimulationCOS CellsEndosomesErbB ReceptorsGreen Fluorescent ProteinsLasersMicroscopy, FluorescenceMolecular BiologyMolecular ImagingPhotobleachingProtein MultimerizationAdaptor Proteins, Signal TransducingAPPL1 protein, humanEGFR protein, humanErbB ReceptorsGreen Fluorescent Proteins

Identifiers

PMID28071667
PMCPMC5234080
OpenAlexW2495439732

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.