ArticleThe Journal of cell biology2017
A targeted RNAi screen identifies factors affecting diverse stages of receptor-mediated transcytosis.
Article in The Journal of cell biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- The Genetic Architecture of Congenital Diarrhea and Enteropathy.The New England journal of medicine · 2025Article
- The PAR6B-PRKCI-PAR3 complex influences alveolar regeneration in patients with the emphysema subtype of chronic obstructive pulmonary disease.Stem cell research & therapy · 2025Article
- Whole-body deletion of Endospanin 1 protects from obesity-associated deleterious metabolic alterations.JCI insight · 2024Article
- Albumin uptake and processing by the proximal tubule: physiological, pathological, and therapeutic implications.Physiological reviews · 2022Review
- Depletion of the apical endosome in response to viruses and bacterial toxins provides cell-autonomous host defense at mucosal surfaces.Cell host & microbe · 2022Article
- Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays.Fluids and barriers of the CNS · 2021Article
- Cholera Toxin as a Probe for Membrane Biology.Toxins · 2021Review
- Is LRP2 Involved in Leptin Transport over the Blood-Brain Barrier and Development of Obesity?International journal of molecular sciences · 2021Article
- Differentiating Between Tight Junction-Dependent and Tight Junction-Independent Intestinal Barrier Loss In Vivo.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The neonatal Fc receptor in cancer FcRn in cancer.Cancer medicine · 2020Review
- Transcytosis maintains CFTR apical polarity in the face of constitutive and mutation-induced basolateral missorting.Journal of cell science · 2019Article
- Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells.Bio-protocol · 2018Article
- Targeting FcRn to Generate Antibody-Based Therapeutics.Trends in pharmacological sciences · 2018Review
- Advances in Evaluation of Chronic Diarrhea in Infants.Gastroenterology · 2018Review
- Mucosal absorption of therapeutic peptides by harnessing the endogenous sorting of glycosphingolipids.eLife · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Endosome transport by transcytosis is the primary mechanism by which proteins and other large cargo traverse epithelial barriers in normal tissue. Transcytosis is also essential for establishing and maintaining membrane polarity in epithelia and other polarized cells. To identify novel components of this pathway, we conducted a high-throughput RNA interference screen for factors necessary for the bidirectional transcytosis of IgG by the Fcγ receptor FcRn. This screen identified 23 genes whose suppression resulted in a reproducible decrease in FcRn-mediated transcytosis. Pulse-chase kinetic transport assays on four of the top-ranking genes (EXOC2, EXOC7, PARD6B, and LEPROT) revealed distinct effects on the apical and basolateral recycling and transcytotic pathways, demonstrating that these pathways are genetically separable. We also found a strong dependence on PARD6B for apical, but not basolateral, recycling, implicating this cell polarity gene in assembly or maintenance of the apical endosomal system. This dataset yields insights into how vesicular transport is adapted to the specialized functions of differentiated cell types and opens new research avenues into epithelial trafficking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.