ArticleAnalytical biochemistry2017
Determination of extinction coefficients of human hemoglobin in various redox states.
Article in Analytical biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 115 citations in OpenAlex.
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- Heme as a Pro-Inflammatory Stimulus in Abdominal Aortic Aneurysm.Antioxidants (Basel, Switzerland) · 2026Article
- On the Effect of Melittin on Surface Properties of Erythrocyte and Mitochondrial Membranes.Membranes · 2025Article
- Biosensors for Detection of Labile Heme in Biological Samples.Biosensors · 2025Review
- A Novel Nano-Scale Biosensor for Measuring Hemoglobin Oxygen Saturation Using Carbon Quantum Dots.Micromachines · 2025Article
- Assessing and monitoring abnormal retinal blood circulation for early detection of pre-structural damage and enhanced diabetic retinopathy staging using non-invasive, high-resolution biophotonic imaging technology.Eye (London, England) · 2025Review
- Redox Potential of Hemoglobin Sub-Micron Particles and Impact of Layer-by-Layer Coating.International journal of molecular sciences · 2025Article
- Inhibiting heme piracy by pathogenic Escherichia coli using de novo-designed proteins.Nature communications · 2025Article
- Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using blot-free vitrification.Nature communications · 2025Article
- Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation.Scientific reports · 2024Article
- Hemoglobin in Submicron Particles (HbMPs) Is Stabilized Against Oxidation.Antioxidants (Basel, Switzerland) · 2024Article
- Article
- Dual excitation spectral autofluorescence lifetime and reflectance imaging for fast macroscopic characterization of tissues.Biomedical optics express · 2024Article
- Biopreservation and Reversal of Oxidative Injury During Blood Storage by a Novel Curcumin-based Gel Formulation.Research square · 2024Article
- Reagentless Vis-NIR Spectroscopy Point-of-Care for Feline Total White Blood Cell Counts.Biosensors · 2024Article
- Berberine mediated fluorescent gold nanoclusters in biomimetic erythrocyte ghosts as a nanocarrier for enhanced photodynamic treatment.RSC advances · 2024Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The role of hemoglobin (Hb) redox forms in tissue and organ toxicities remain ambiguous despite the well-documented contribution of Hb redox reactivity to cellular and subcellular oxidative changes. Moreover, several recent studies, in which Hb toxicity were investigated, have shown conflicting outcomes. Uncertainties over the potential role of these species may in part be due to the protein preparation method of choice, the use of published extinction coefficients and the lack of suitable controls for Hb oxidation and heme loss. Highly purified and well characterized redox forms of human Hb were used in this study and the extinction coefficients of each Hb species (ferrous/oxy, ferric/met and ferryl) were determined. A new set of equations were established to improve accuracy in determining the transient ferryl Hb species. Additionally, heme concentrations in solutions and in human plasma were determined using a novel reversed phase HPLC method in conjugation with our photometric measurements. The use of more accurate redox-specific extinction coefficients and method calculations will be an invaluable tool for both in vitro and in vivo experiments aimed at determining the role of Hb-mediated vascular pathology in hemolytic anemias and when Hb is used as oxygen therapeutics.
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Registered trials
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