ArticleBMC genomics2017
Functional changes in mRNA expression and alternative pre-mRNA splicing associated with the effects of nutrition on apoptosis and spermatogenesis in the adult testis.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Comparison of Sertoli cells in duck testes pre- and post-sexual maturation.Poultry science · 2026Article
- Review
- The Impact ofAnimals : an open access journal from MDPI · 2025Article
- Identification and Functional Analysis of PANoptosis-Associated Genes in the Progression From Sepsis to ARDS.Immunity, inflammation and disease · 2025Article
- Article
- Folic acid and sperm quality improvement: insights from snRNA sequencing and RNA splicing mechanisms.Frontiers in nutrition · 2025Review
- CARF regulates the alternative splicing and piwi/piRNA complexes during mouse spermatogenesis through PABPC1.Acta biochimica et biophysica Sinica · 2024Article
- A Single-Step Genome-Wide Association Study for Semen Traits of Egyptian Buffalo Bulls.Animals : an open access journal from MDPI · 2023Article
- Novel Aspects of cAMP-Response Element Modulator (CREM) Role in Spermatogenesis and Male Fertility.International journal of molecular sciences · 2023Review
- Identification and Functional Assignment of Genes Implicated in Sperm Maturation of Tibetan Sheep.Animals : an open access journal from MDPI · 2023Article
- Paternal effects on fetal programming.Animal reproduction · 2023Review
- Characterization of Alternative Splicing (AS) Events during Chicken (Animals : an open access journal from MDPI · 2021Article
- Identification of Key Genes Related to Postnatal Testicular Development Based on Transcriptomic Data of Testis in Hu Sheep.Frontiers in genetics · 2021Article
- Genome-wide association study to identify genomic regions and positional candidate genes associated with male fertility in beef cattle.Scientific reports · 2020Article
- Spliced genes in muscle from Nelore Cattle and their association with carcass and meat quality.Scientific reports · 2020Article
- Effect of lesion proximity on the regenerative response of long descending propriospinal neurons after spinal transection injury.BMC neuroscience · 2019Article
- Palmitate- and C6 ceramide-inducedNutrition & metabolism · 2018Article
- Long noncoding RNA expression profile changes associated with dietary energy in the sheep testis during sexual maturation.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe effects of nutrition on testis mass in the sexually mature male have long been known, however, the cellular and molecular processes of the testis response to nutrition was not fully understood.
methodsWe tested whether the defects in spermatogenesis and increases in germ cell apoptosis in the testis that are induced by under-nutrition are associated with changes in mRNA expression and pre-mRNA alternative splicing using groups of 8 male sheep fed for a 10% increase or 10% decrease in body mass over 65 days.
resultsWe identified 2,243 mRNAs, including TP53 and Claudin 11, that were differentially expressed in testis from underfed and well-fed sheep (FDR < 0.1), and found that their expression changed in parallel with variations in germ cell numbers, testis size, and spermatogenesis. Furthermore, pairs of 269 mRNAs and 48 miRNAs were identified on the basis of target prediction. The regulatory effect of miRNAs on mRNA expression, in combination with functional analysis, suggests that these miRNAs are involved in abnormal reproductive morphology, apoptosis and male infertility. Nutrition did not affect the total number of alternative splicing events, but affected 206 alternative splicing events. A total of 159 genes, including CREM, SPATA6, and DDX4, were differentially spliced between dietary treatments, with functions related to RNA splicing and spermatogenesis. In addition, three gene modules were positively correlated with spermatogenesis-related phenotypic traits and negatively related to apoptosis-related phenotypic traits. Among these gene modules, seven (CFLAR, PTPRC, F2R, MAP3K1, EPHA7, APP, BCAP31) were also differentially expressed between nutritional treatments, indicating their potential as markers of spermatogenesis or apoptosis.
conclusionsOur findings on significant changes in mRNAs and pre-mRNA alternative splicing under-nutrition suggest that they may partly explain the disruption of spermatogenesis and the increase germ cell apoptosis. However, more research is required to verify their causal effects in regulating spermatogenesis and germ cell apoptosis.
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