ArticleNature methods2017
Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging.
Article in Nature methods, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
What it found
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Who cites it
86 citing papers in PubMed.
- An Open, Integrated Platform for Multiplexed Bioluminescence Microscopy.Chemical & biomedical imaging · 2026Article
- Label-free biochemical imaging and time point analysis of neural organoids via deep learning-enhanced Raman microspectroscopy.Science advances · 2026Article
- Structural, Compositional, and Dielectric State Profiling in Label-Free Single-Cell Monitoring.Small methods · 2026Review
- A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales.eLife · 2026Article
- Resolving fluorescently labeled species using highly multiplexed spectral FLIM.Scientific reports · 2026Article
- Comprehensive and Region-Specific Retinal Health Assessment Using Phasor Analysis of Multispectral Images and Machine Learning.Sensors (Basel, Switzerland) · 2026Article
- Redefining colocalization analysis with a novel phasor mixing coefficient.Journal of cell science · 2026Article
- Multiplexed Integrin Detection and Cancer Cell Classification Using Multicolor Gap-Enhanced Gold Nanorods and Machine Learning Algorithm.Nanomaterials (Basel, Switzerland) · 2025Article
- ESPRESSO: spatiotemporal omics based on organelle phenotyping.Nature methods · 2025Article
- Transforming hyperspectral data into insight: the DREAM approach for pathology.Biomedical optics express · 2025Article
- Article
- Spectral phasor imaging on a commercial confocal microscope without a spectral detector.Scientific reports · 2025Article
- Quantifying uncertainty in phasor-based time-domain fluorescence lifetime imaging microscopy.Biomedical optics express · 2025Article
- PolSpec : Polarisation-Based Detection for Versatile, Cost-Effective Rapid Hyperspectral Imaging.Journal of biophotonics · 2025Article
- Periodic Light Modulations for Low-Cost Wide-Field Imaging of Luminescence Kinetics Under Ambient Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Multispectral live-cell imaging with uncompromised spatiotemporal resolution.Nature photonics · 2025Article
- Unsupervised inter-domain transformation for virtually stained high-resolution mid-infrared photoacoustic microscopy using explainable deep learning.Nature communications · 2024Article
- Highly multiplexed fluorescence microscopy with spectrally tunable semiconducting polymer dots.Science advances · 2024Article
- SpectraTrack: megapixel, hundred-fps, and thousand-channel hyperspectral imaging.Nature communications · 2024Article
- Supercontinuum-tailoring multicolor imaging reveals spatiotemporal dynamics of heterogeneous tumor evolution.Nature communications · 2024Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Time-lapse imaging of multiple labels is challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quality, while throughput can be limited by processing time. Here, we report software called Hyper-Spectral Phasors (HySP) for denoising and unmixing multiple spectrally overlapping fluorophores in a low signal-to-noise regime with fast analysis. We show that HySP enables unmixing of seven signals in time-lapse imaging of living zebrafish embryos.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.