ArticleScientific reports2017
The mechanism of catalysis by type-II NADH:quinone oxidoreductases.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 71 citations in OpenAlex.
- Quinone-transporting filaments expand bioenergetic capacity in Gram-positive Bacillota.Nature microbiology · 2026Article
- Structural basis of ligand selectivity in FAD/NAD(P)H-dependent dehydrogenases: insights from trypanothione reductase and type II NADH dehydrogenase.Protein science : a publication of the Protein Society · 2026Article
- Selective inhibition of respiratory complex I reveals a bioenergetic vulnerability inbioRxiv : the preprint server for biology · 2026Article
- Genomic heterogeneity of NAD(P)H dehydrogenase predisposes Cryptosporidium to clofazimine resistance.Nature microbiology · 2026Article
- Free Radical Formation in the Reactions of Redox-Active Drugs and Xenobiotics with Mitochondrial Flavoenzymes.Biomolecules · 2026Review
- α-Mangostin Competing the Menaquinone-Binding Sites of NDH-2 to Block the Electron Transfer at the Quinone Pool ofAntibiotics (Basel, Switzerland) · 2026Article
- The heme A synthase Cox15, as a target of redox-active 3-benzylmenadiones with antiparasitic activity.Antimicrobial agents and chemotherapy · 2026Article
- Widespread genomic heterogeneity at the type II NAD(P)H dehydrogenase locus predisposesbioRxiv : the preprint server for biology · 2025Article
- Naphthoquinone preferentially pairs with non-proton-pumping NADH dehydrogenase for respiratory electron transport.PLoS genetics · 2025Article
- Protein-ligand co-design: a case for improving binding affinity between type II NADH:quinone oxidoreductase and quinones.Journal of computer-aided molecular design · 2025Article
- Identifying Key Properties That Drive Redox Mediator Activity in Lactiplantibacillus Plantarum.Angewandte Chemie (International ed. in English) · 2025Article
- Reactions ofInternational journal of molecular sciences · 2025Article
- Modulating Liposome Surface Charge for Maximized ATP Regeneration in Synthetic Nanovesicles.ACS synthetic biology · 2024Article
- Effect of Light Regime onJournal of microbiology and biotechnology · 2024Article
- A respiro-fermentative strategy to survive nanoxia in Acidobacterium capsulatum.FEMS microbiology ecology · 2024Article
- Review
- Quantitative proteomics reveals oxygen-induced adaptations inFrontiers in microbiology · 2024Article
- Structural insights into FSP1 catalysis and ferroptosis inhibition.Nature communications · 2023Article
- Article
- A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase.Nature communications · 2023Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
Type II NADH:quinone oxidoreductase (NDH-2) is central to the respiratory chains of many organisms. It is not present in mammals so may be exploited as an antimicrobial drug target or used as a substitute for dysfunctional respiratory complex I in neuromuscular disorders. NDH-2 is a single-subunit monotopic membrane protein with just a flavin cofactor, yet no consensus exists on its mechanism. Here, we use steady-state and pre-steady-state kinetics combined with mutagenesis and structural studies to determine the mechanism of NDH-2 from Caldalkalibacillus thermarum. We show that the two substrate reactions occur independently, at different sites, and regardless of the occupancy of the partner site. We conclude that the reaction pathway is determined stochastically, by the substrate/product concentrations and dissociation constants, and can follow either a ping-pong or ternary mechanism. This mechanistic versatility provides a unified explanation for all extant data and a new foundation for the development of therapeutic strategies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.