ArticleJournal of tissue engineering and regenerative medicine2017
Developing a biomimetic tooth bud model.
Article in Journal of tissue engineering and regenerative medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- A Fully Defined GelMA-Based Matrix Allows Fine Tuning of Tissue-Relevant Biomechanical and Biochemical Cues for Organoid Culture.Advanced healthcare materials · 2026Article
- Oral tissue spheroid, organoid, and organ-on chip microphysiological modeling strategies towards enhanced emulation of health and disease.Bioengineering & translational medicine · 2025Review
- Oral Implantology-Current Concepts.Journal of maxillofacial and oral surgery · 2025Article
- Organoids in the oral and maxillofacial region: present and future.International journal of oral science · 2024Review
- Engineered organoids in oral and maxillofacial regeneration.iScience · 2023Review
- Bioprinting and biomaterials for dental alveolar tissue regeneration.Frontiers in bioengineering and biotechnology · 2023Review
- Towards a New Concept of Regenerative Endodontics Based on Mesenchymal Stem Cell-Derived Secretomes Products.Bioengineering (Basel, Switzerland) · 2022Review
- A Compilation of Study Models for Dental Pulp Regeneration.International journal of molecular sciences · 2022Review
- Stem cells and common biomaterials in dentistry: a review study.Journal of materials science. Materials in medicine · 2022Review
- Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review.Gels (Basel, Switzerland) · 2022Review
- Self-Assembled Hydrogel Microparticle-Based Tooth-Germ Organoids.Bioengineering (Basel, Switzerland) · 2022Article
- A critical analysis of research methods and biological experimental models to study pulp regeneration.International endodontic journal · 2022Review
- 3D-bioprinted Recombination Structure of Hertwig's Epithelial Root Sheath Cells and Dental Papilla Cells for Alveolar Bone Regeneration.International journal of bioprinting · 2022Article
- Advanced in Vitro Experimental Models for Tissue Engineering-based Reconstruction of a 3D Dentin/pulp Complex: a Literature Review.Stem cell reviews and reports · 2021Review
- Hard Dental Tissues Regeneration-Approaches and Challenges.Materials (Basel, Switzerland) · 2021Review
- Tooth Repair and Regeneration: Potential of Dental Stem Cells.Trends in molecular medicine · 2021Review
- Scaffold-based developmental tissue engineering strategies for ectodermal organ regeneration.Materials today. Bio · 2021Review
- FACEts of mechanical regulation in the morphogenesis of craniofacial structures.International journal of oral science · 2021Review
- Tissue Engineering in Stomatology: A Review of Potential Approaches for Oral Disease Treatments.Frontiers in bioengineering and biotechnology · 2021Review
- Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts.Journal of clinical medicine · 2019Review
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Authors and funding
6 authors.
Funding
Abstract
A long-term goal is to bioengineer, fully functional, living teeth for regenerative medicine and dentistry applications. Biologically based replacement teeth would avoid insufficiencies of the currently used dental implants. Using natural tooth development as a guide, a model was fabricated using post-natal porcine dental epithelial (pDE), porcine dental mesenchymal (pDM) progenitor cells, and human umbilical vein endothelial cells (HUVEC) encapsulated within gelatin methacrylate (GelMA) hydrogels. Previous publications have shown that post-natal DE and DM cells seeded onto synthetic scaffolds exhibited mineralized tooth crowns composed of dentin and enamel. However, these tooth structures were small and formed within the pores of the scaffolds. The present study shows that dental cell-encapsulated GelMA constructs can support mineralized dental tissue formation of predictable size and shape. Individually encapsulated pDE or pDM cell GelMA constructs were analysed to identify formulas that supported pDE and pDM cell attachment, spreading, metabolic activity, and neo-vasculature formation with co-seeded endothelial cells (HUVECs). GelMa constructs consisting of pDE-HUVECS in 3% GelMA and pDM-HUVECs within 5% GelMA supported dental cell differentiation and vascular mineralized dental tissue formation in vivo. These studies are the first to demonstrate the use of GelMA hydrogels to support the formation of post-natal dental progenitor cell-derived mineralized and functionally vascularized tissues of specified size and shape. These results introduce a novel three-dimensional biomimetic tooth bud model for eventual bioengineered tooth replacement teeth in humans. Copyright © 2017 John Wiley & Sons, Ltd.
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