ArticleBiotechnology for biofuels2017
Rapid in vivo lipid/carbohydrate quantification of single microalgal cell by Raman spectral imaging to reveal salinity-induced starch-to-lipid shift.
Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Influence of salinity, nitrogen and phosphorus concentrations on the physiological and biochemical characteristics of two Chlorophyceae isolated from Fez freshwater, Morocco.Scientific reports · 2024Article
- Potential use of saline resources for biofuel production using halophytes and marine algae: prospects and pitfalls.Frontiers in plant science · 2023Review
- Adsorption Analysis of Exopolymeric Substances as a Tool for the Materials Selection of Photobioreactors Manufacture.International journal of molecular sciences · 2022Article
- Review
- Raman Scattering-Based Biosensing: New Prospects and Opportunities.Biosensors · 2021Review
- Article
- Mapping Single Walled Carbon Nanotubes in Photosynthetic Algae by Single-Cell Confocal Raman Microscopy.Materials (Basel, Switzerland) · 2020Article
- Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells.Nature biotechnology · 2020Article
- Lipids monitoring inBiotechnology for biofuels · 2020Article
- Parallelized shifted-excitation Raman difference spectroscopy for fluorescence rejection in a temporary varying system.Journal of biophotonics · 2019Article
- Determination of hydroxyl groups in biorefinery resources via quantitativeNature protocols · 2019Article
- FTIR and Raman Spectroscopy-Based Biochemical Profiling Reflects Genomic Diversity of ClinicalInternational journal of molecular sciences · 2019Article
- An Expandable Mechanopharmaceutical Device (3): a Versatile Raman Spectral Cytometry Approach to Study the Drug Cargo Capacity of Individual Macrophages.Pharmaceutical research · 2018Article
- Single cell assessment of yeast metabolic engineering for enhanced lipid production using Raman and AFM-IR imaging.Biotechnology for biofuels · 2018Article
- Label-free, simultaneous quantification of starch, protein and triacylglycerol in single microalgal cells.Biotechnology for biofuels · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLipid/carbohydrate content and ratio are extremely important when engineering algal cells for liquid biofuel production. However, conventional methods for such determination and quantification are not only destructive and tedious, but also energy consuming and environment unfriendly. In this study, we first demonstrate that Raman spectroscopy is a clean, fast, and accurate method to simultaneously quantify the lipid/carbohydrate content and ratio in living microalgal cells.
resultsThe quantification results of both lipids and carbohydrates obtained by Raman spectroscopy showed a linear correspondence with that obtained by conventional methods, indicating Raman can provide a similar accuracy to conventional methods, with a significantly shorter detection time. Furthermore, the subcellular resolution of Raman spectroscopy enabled not only the concentration mapping of lipid/carbohydrate content in single living cells, but also the evaluation of standard deviation between the biomass accumulation levels of individual algal cells.
conclusionsIn this study, we first demonstrate that Raman spectroscopy can be used for starch quantification in addition to lipid quantification in algal cells. Due to the easiness and non-destructive nature of Raman spectroscopy, it makes a perfect tool for the further study of starch-lipid shift mechanism.
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