ArticleFrontiers in plant science2016
A Benchtop Fractionation Procedure for Subcellular Analysis of the Plant Metabolome.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Thermo-flux: generation and analysis of thermodynamic-stoichiometric metabolic network models.Molecular systems biology · 2026Article
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- Illuminating the subcellular maze: fluorescence-activated organelle sorting in plant sciences.Journal of experimental botany · 2025Review
- Subcellular plant carbohydrate metabolism under elevated temperature.Plant physiology · 2025Article
- Insights into physiological roles of flavonoids in plant cold acclimation.The Plant journal : for cell and molecular biology · 2024Article
- Symplasmic phloem loading and subcellular transport in storage roots are key factors for carbon allocation in cassava.Plant physiology · 2024Article
- Can plant hormonomics be built on simple analysis? A review.Plant methods · 2023Review
- The SnRK2.3-AREB1-TST1/2 cascade activated by cytosolic glucose regulates sugar accumulation across tonoplasts in apple and tomato.Nature plants · 2023Article
- Retrograde signaling in plants: A critical review focusing on the GUN pathway and beyond.Plant communications · 2023Review
- Impaired cell growth under ammonium stress explained by modeling the energy cost of vacuole expansion in tomato leaves.The Plant journal : for cell and molecular biology · 2022Article
- "Omics" insights into plastid behavior toward improved carotenoid accumulation.Frontiers in plant science · 2022Review
- Metabolic regulation of subcellular sucrose cleavage inferred from quantitative analysis of metabolic functions.Quantitative plant biology · 2022Review
- In Vivo NADH/NADThe Plant cell · 2020Article
- Activities of leaf and spike carbohydrate-metabolic and antioxidant enzymes are linked with yield performance in three spring wheat genotypes grown under well-watered and drought conditions.BMC plant biology · 2020Article
- Tracing metabolic flux through time and space with isotope labeling experiments.Current opinion in biotechnology · 2020Review
- Differential Contribution of P5CS Isoforms to Stress Tolerance in Arabidopsis.Frontiers in plant science · 2020Article
- Resolving subcellular plant metabolism.The Plant journal : for cell and molecular biology · 2019Article
- Dynamics of Plant Metabolism during Cold Acclimation.International journal of molecular sciences · 2019Review
- Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments ofBio-protocol · 2019Article
- The Plastidic Sugar Transporter pSuT Influences Flowering and Affects Cold Responses.Plant physiology · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Although compartmentation is a key feature of eukaryotic cells, biological research is frequently limited by methods allowing for the comprehensive subcellular resolution of the metabolome. It has been widely accepted that such a resolution would be necessary in order to approximate cellular biochemistry and metabolic regulation, yet technical challenges still limit both the reproducible subcellular fractionation and the sample throughput being necessary for a statistically robust analysis. Here, we present a method and a detailed protocol which is based on the non-aqueous fractionation technique enabling the assignment of metabolites to their subcellular localization. The presented benchtop method aims at unraveling subcellular metabolome dynamics in a precise and statistically robust manner using a relatively small amount of tissue material. The method is based on the separation of cellular fractions via density gradients consisting of organic, non-aqueous solvents. By determining the relative distribution of compartment-specific marker enzymes together with metabolite profiles over the density gradient it is possible to estimate compartment-specific metabolite concentrations by correlation. To support this correlation analysis, a spreadsheet is provided executing a calculation algorithm to determine the distribution of metabolites over subcellular compartments. The calculation algorithm performs correlation of marker enzyme activity and metabolite abundance accounting for technical errors, reproducibility and the resulting error propagation. The method was developed, tested and validated in three natural accessions of
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.