ArticleFrontiers in microbiology2016
16S Based Microbiome Analysis from Healthy Subjects' Skin Swabs Stored for Different Storage Periods Reveal Phylum to Genus Level Changes.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 66 citations in OpenAlex.
- A Microbial Signature of Psychological Distress in Irritable Bowel Syndrome.Psychosomatic medicine · 2018Trial
- The Influence of Proton Pump Inhibitors on the Fecal Microbiome of Infants with Gastroesophageal Reflux-A Prospective Longitudinal Interventional Study.Frontiers in cellular and infection microbiology · 2017Trial
- The Fecal Microbiome of IBD Patients Is Less Divertible by Bowel Preparation Compared to Healthy Controls: Results From a Prospective Study.Inflammatory bowel diseases · 2025Article
- Optimisation of cutaneous microbiota sampling methodology.Frontiers in microbiomes · 2025Article
- In-hospital areas with distinct maintenance and staff/patient traffic have specific microbiome profiles, functions, and resistomes.mSystems · 2024Article
- Rapid biotransformation of STW 5 constituents by human gut microbiome from IBS- and non-IBS donors.Microbiology spectrum · 2024Article
- Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2024Article
- Reduced olfactory performance is associated with changed microbial diversity, oralization, and accumulation of dead biomaterial in the nasal olfactory area.Microbiology spectrum · 2024Article
- Clinical NEC prevention practices drive different microbiome profiles and functional responses in the preterm intestine.Nature communications · 2023Article
- Effect of Antibiotic Eye Drops on the Nasal Microbiome in Healthy Subjects-A Pilot Study.Antibiotics (Basel, Switzerland) · 2023Article
- Probiotic Supplementation Attenuates Chemotherapy-Induced Intestinal Mucositis in an Experimental Colorectal Cancer Liver Metastasis Rat Model.Nutrients · 2023Article
- A large scale 16S ribosomal RNA gene amplicon dataset of hand, foot and mouth patients and healthy individuals.Scientific data · 2023Article
- Towards standardized and reproducible research in skin microbiomes.Environmental microbiology · 2022Review
- Genetic and phenotypic diversity of fecal Candida albicans strains in irritable bowel syndrome.Scientific reports · 2022Article
- Insights into the microbial life in silica-rich subterranean environments: microbial communities and ecological interactions in an orthoquartzite cave (Imawarì Yeuta, Auyan Tepui, Venezuela).Frontiers in microbiology · 2022Article
- Reduced B12 uptake and increased gastrointestinal formate are associated with archaeome-mediated breath methane emission in humans.Microbiome · 2021Article
- Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study.BMC microbiology · 2021Article
- Transition from unclassified Ktedonobacterales to Actinobacteria during amorphous silica precipitation in a quartzite cave environment.Scientific reports · 2021Article
- Human Milk Oligosaccharides Modulate the Risk for Preterm Birth in a Microbiome-Dependent and -Independent Manner.mSystems · 2020Article
- Insights into the gut microbiota of Nigerian elderly with type 2 diabetes and non-diabetic elderly persons.Heliyon · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbiome research and improvements in high throughput sequencing technologies revolutionize our current scientific viewpoint. The human associated microbiome is a prominent focus of clinical research. Large cohort studies are often required to investigate the human microbiome composition and its changes in a multitude of human diseases. Reproducible analyses of large cohort samples require standardized protocols in study design, sampling, storage, processing, and data analysis. In particular, the effect of sample storage on actual results is critical for reproducibility. So far, the effect of storage conditions on the results of microbial analysis has been examined for only a few human biological materials (e.g., stool samples). There is a lack of data and information on appropriate storage conditions on other human derived samples, such as skin. Here, we analyzed skin swab samples collected from three different body locations (forearm, V of the chest and back) of eight healthy volunteers. The skin swabs were soaked in sterile buffer and total DNA was isolated after freezing at -80°C for 24 h, 90 or 365 days. Hypervariable regions V1-2 were amplified from total DNA and libraries were sequenced on an Illumina MiSeq desktop sequencer in paired end mode. Data were analyzed using Qiime 1.9.1. Summarizing all body locations per time point, we found no significant differences in alpha diversity and multivariate community analysis among the three time points. Considering body locations separately significant differences in the richness of forearm samples were found between d0 vs. d90 and d90 vs. d365. Significant differences in the relative abundance of major skin genera (
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