Evidence map›Paper›PMID 28065656›Full record

ArticleCell chemical biology2017

APT2 Inhibition Restores Scribble Localization and S-Palmitoylation in Snail-Transformed Cells.

Jeannie L Hernandez, Dahvid Davda, Melanie Cheung See Kit, Jaimeen D Majmudar, Sang Joon Won, Margery Gang, Sirisha C Pasupuleti, Alexandria I Choi, Callie M Bartkowiak, Brent R Martin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.

0numbers the graph read from it
0cells of the map it votes in
52citing papers in PubMed
4.4field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

52 citing papers in PubMed, 80 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. A novel approach to combatMicrobiology spectrum · 2026
    Article
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Enzyme-Responsive Peptide Thioesters for Targeting Golgi Apparatus.Journal of the American Chemical Society · 2022
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Jeannie L HernandezDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Dahvid DavdaDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA; Program in Chemical Biology, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Melanie Cheung See KitDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Jaimeen D MajmudarDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Sang Joon WonProgram in Chemical Biology, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Margery GangDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Sirisha C PasupuletiDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Alexandria I ChoiDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Callie M BartkowiakDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA.
Brent R MartinDepartment of Chemistry, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA; Program in Chemical Biology, University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109, USA. Electronic address: brentrm@umich.edu.
University of Michigan–Ann Arbor · US

Funding

Multiscale chemical approaches to map oxidative stressDP2GM114848 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MARTIN, BRENT RANDALL · 2014 to 2014
$2.2M
Quantitative chemical proteomics of dynamic palmitoylation in cellsR00CA151460 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MARTIN, BRENT RANDALL · 2011 to 2013
$769k
NCI NIH HHS R00 CA151460NIGMS NIH HHS DP2 GM114848
6 · The paper itself

Abstract

The multidomain scaffolding protein Scribble (Scrib) organizes key signaling complexes to specify basolateral cell polarity and suppress aberrant growth. In many human cancers, genetically normal Scrib mislocalizes from cell-cell junctions to the cytosol, correlating with enhanced growth signaling and malignancy. Here we confirm that expression of the epithelial-to-mesenchymal transcription factor (EMT-TF) Snail in benign epithelial cells leads to Scrib displacement from the plasma membrane, mimicking the mislocalization observed in aggressive cancers. Upon further examination, Snail promotes a transcriptional program that targets genes in the palmitoylation cycle, repressing many protein acyl transferases and elevating expression and activity of protein acyl thioesterase 2 (APT2). APT2 isoform-selective inhibition or knockdown rescued Scrib membrane localization and palmitoylation while attenuating MEK activation. Overall, inhibiting APT2 restores balance to the Scrib palmitoylation cycle, promoting membrane re-localization and growth attenuation. These findings emphasize the importance of S-palmitoylation as a post-translational gatekeeper of cell polarity-mediated tumor suppression.

Indexed as

Cell MembraneCells, CulturedEpithelial CellsEpithelial-Mesenchymal TransitionHumansLipoylationMembrane ProteinsSnail Family Transcription FactorsThiolester HydrolasesTumor Suppressor ProteinsLYPLA2 protein, humanMembrane ProteinsSCRIB protein, humanSnail Family Transcription FactorsThiolester HydrolasesTumor Suppressor Proteinsactivity-based profilingcell polarityepithelial-mesenchymal transitionfluorescence imaginghydrolaseinhibitorprotein palmitoylation

Identifiers

PMID28065656
PMCPMC5362123
OpenAlexW2569029581

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.