ArticleCell chemical biology2017
Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System.
Article in Cell chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Article
- Modulating Protein Function through Genetically Encoded Oxidative Chemistry.Journal of the American Chemical Society · 2026Article
- Cancer Neoaxonogenesis: Mechanisms and Factors Involved in the Recruitment of Peripheral Nerves by Cancer Tissue.International journal of molecular sciences · 2026Review
- A brief guide to the in vivo chemogenetic cell ablation approach in zebrafish.Molecules and cells · 2026Article
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
- Tumor Innervation: From Bystander to Emerging Therapeutic Target for Cancer.International journal of molecular sciences · 2025Review
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Proximitomics by Reactive Species.ACS central science · 2024Review
- Towards overcoming obstacles of type II photodynamic therapy: Endogenous production of light, photosensitizer, and oxygen.Acta pharmaceutica Sinica. B · 2024Review
- Article
- Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Imaginal Disc Regeneration: Something Old, Something New.Cold Spring Harbor perspectives in biology · 2022Review
- Article
- Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling.Nature communications · 2022Article
- Optogenetics at the presynapse.Nature neuroscience · 2022Review
- Article
- A single motor neuron determines the rhythm of early motor behavior inScience advances · 2021Article
- Genetically Encoded Photosensitizer for Destruction of Protein or Cell Function.Advances in experimental medicine and biology · 2021Review
- Genetically Encoded Red Photosensitizers with Enhanced Phototoxicity.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva. In addition, miniSOG2 is able to photoablate a small group of cells in one of the larval wing imaginal discs, resulting in an adult with one incomplete and one normal wing. We expect miniSOG2 to be a useful optogenetic tool for precision cell ablation at a desired developmental time point in live animals, thus opening a new window into cell origin, fate and function, tissue regeneration, and developmental biology.
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Registered trials
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