ArticleToxicology2017
Comparison of fluorescence-based methods to determine nanoparticle uptake by phagocytes and non-phagocytic cells in vitro.
Article in Toxicology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- Quantifying Cellular Uptake of NanoplasticsACS omega · 2026Article
- Size-Dependent Immunomodulatory Effects of FeMolecules (Basel, Switzerland) · 2026Article
- Force Spectroscopy by Atomic Force Microscopy as Indicator for Cellular Microplastic Uptake.International journal of molecular sciences · 2026Article
- Extracellular Vesicles and PlantCrystals for Improved Bioavailability of Curcumin as a BCS Class IV Drug.Molecules (Basel, Switzerland) · 2024Article
- Unveiling Nanoparticles: Recent Approaches in Studying the Internalization Pattern of Iron Oxide Nanoparticles in Mono- and Multicellular Biological Structures.Journal of functional biomaterials · 2024Review
- Uptake Quantification of Antigen Carried by Nanoparticles and Its Impact on Carrier Adjuvanticity Evaluation.Vaccines · 2023Article
- Antioxidant Defense in Primary Murine Lung Cells following Short- and Long-Term Exposure to Plastic Particles.Antioxidants (Basel, Switzerland) · 2023Article
- Influence of Cell Type on the Efficacy of Plasmonic Photothermal Therapy.ACS nanoscience Au · 2022Article
- Quantifying fluorescent nanoparticle uptake in mammalian cells using a plate reader.Scientific reports · 2022Article
- Physiologically based pharmacokinetic modeling of intravenously administered nanoformulated substances.Drug delivery and translational research · 2022Article
- Modified Stability of microRNA-Loaded Nanoparticles.Pharmaceutics · 2022Article
- Designing Functional Bionanoconstructs for EffectiveBioconjugate chemistry · 2022Review
- Pharmacokinetics of PEGylated Gold Nanoparticles: In Vitro-In Vivo Correlation.Nanomaterials (Basel, Switzerland) · 2022Article
- Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Numerical analysis of the impact of cytoskeletal actin filament density alterations onto the diffusive vesicle-mediated cell transport.PLoS computational biology · 2021Article
- Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure.Nanomaterials (Basel, Switzerland) · 2021Article
- Labeling PLA-PEG nanocarriers with IR780: physical entrapment versus covalent attachment to polylactide.Drug delivery and translational research · 2020Article
- Distinct Proteins in Protein Corona of Nanoparticles Represent a Promising Venue for Endogenous Targeting - Part II: In vitro and in vivo Kinetics Study.International journal of nanomedicine · 2020Article
- An invisible private 2D barcode design and implementation with tunable fluorescent nanoparticles.RSC advances · 2019Article
- How to Study the Uptake and Toxicity of Nanoparticles in Cultured Brain Cells: The Dos and Don't Forgets.Neurochemical research · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
At many portals of entry the relative uptake by phagocytes and non-phagocytic cells has a prominent effect on availability and biological action of nanoparticles (NPs). Cellular uptake can be determined for fluorescence-labeled NPs. The present study compares three methods (plate reader, flow cytometry and image analysis) in order to investigate the influence of particle size and functionalization and medium content on cellular uptake of fluorescence-labeled polystyrene particles and to study the respective method́s suitability for uptake studies. For comparison between the techniques, ratios of macrophage to alveolar epithelial cell uptakes were used. Presence of serum protein in the exposure solution decreased uptake of carboxyl-functionalized and non-functionalized particles; there was no clear effect for the amine-functionalized particles. The 200nm non- or carboxyl-functionalized NPs were taken up preferentially by phagocytes while for amine-functionalized particles preference was lowest. The presence of the serum slightly increased the preference for these particles. In conclusion, due to the possibility of calibration, plate reader measurements might present a better option than the other techniques to (semi)quantify differences between phagocytes and non-phagocytic cells for particles with different fluorescence. In order to obtain unbiased data the fluorescent labeling has to fulfill certain requirements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.