ArticleJournal of assisted reproduction and genetics2017
Improved detection of mineral oil toxicity using an extended mouse embryo assay.
Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- From mouse embryo assay to bovine embryo assay: towards an ethical and scientifically superior quality control standard in assisted reproduction technologies.Journal of assisted reproduction and genetics · 2026Review
- Impact of mineral oil overlay on immature oocyte maturation in an optimized in vitro culture system.European journal of medical research · 2026Article
- The sequential use of IVF disposable devices doesn't cause cumulative toxicity unless a defective device compromises the entire procedure.Scientific reports · 2026Article
- Environmental Stress-Induced Alterations in Embryo Developmental Morphokinetics.Journal of xenobiotics · 2024Review
- Culture conditions in the IVF laboratory: state of the ART and possible new directions.Journal of assisted reproduction and genetics · 2023Review
- Use of mineral oil in IVF culture systems: physico-chemical aspects, management, and safety.Journal of assisted reproduction and genetics · 2022Review
- Oxidative Stress and Assisted Reproduction: A Comprehensive Review of Its Pathophysiological Role and Strategies for Optimizing Embryo Culture Environment.Antioxidants (Basel, Switzerland) · 2022Review
- The effects of temperature variation treatments on embryonic development: a mouse study.Scientific reports · 2022Article
- Off-gassing plasticware to decrease the toxicity effect in embryo culture.JBRA assisted reproduction · 2021Article
- Mineral oil: safety and use as placebo in REDUCE-IT and other clinical studies.European heart journal supplements : journal of the European Society of Cardiology · 2020Article
- Mouse embryo assay for human in vitro fertilization quality control: a fresh look.Journal of assisted reproduction and genetics · 2020Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSuccessful in vitro fertilization (IVF) relies on sound laboratory methods and culture conditions which depend on sensitive quality control (QC) testing. This study aimed to improve the sensitivity of mouse embryo assays (MEA) for detection of mineral oil toxicity.
methodsFive experiments were conducted to study modifications of the standard mouse embryo assay (MEA) in order to improve sensitivity using clinical grade mineral oil with known peroxide concentrations. Assessment of blastocyst development at either 96 h or in an extended MEA (eMEA) to 144 h was tested in each experiment. In experiment 1, ability to detect peroxides in oil was compared in the MEA, eMEA, and cell number at 96 h. In experiment 2, serial dilutions of peroxide in oil were used along with time-lapse imaging to compare sensitivity of the morphokinetic MEA to the eMEA. Culture conditions that may affect assay sensitivity were assessed in experiments 3-5, which examined the effect of group versus individual culture, oxygen concentration, and protein supplementation.
resultsExtended MEA and cell counts identified toxicity not detected by the routine endpoint of blastocyst rate at 96 h. The eMEA was fourfold more sensitive than the standard MEA, and this sensitivity was similar to the morphokinetic MEA. Group culture had a protective effect against toxicity, while oxygen concentration did not affect blastocyst development. Protein supplementation with HSA had a protective effect on blastocyst development in eMEA.
conclusionsThe standard MEA used by manufacturers does not detect potentially lethal toxicity of peroxides in mineral oil. While group culture may mask toxicity, protein supplementation and oxygen concentration have minimal effect on assay sensitivity. The eMEA and time-lapse morphokinetic assessment are equally effective in detection of peroxide toxicity and thus provide manufacturers and end-users a simple process modification that can be readily adopted into an existing QC program.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.