Evidence map›Paper›PMID 28063091›Full record

ArticleJournal of the American Society for Mass Spectrometry2017

Isolating Protein Charge State Reduction in Electrospray Droplets Using Femtosecond Laser Vaporization.

Santosh Karki, Habiballah Sistani, Jieutonne J Archer, Fengjian Shi, Robert J Levis

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of the American Society for Mass Spectrometry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Quantification of Protein-Ligand Interactions by Laser Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Santosh KarkiDepartment of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
Habiballah SistaniDepartment of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
Jieutonne J ArcherDepartment of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
Fengjian ShiDepartment of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA.
Robert J LevisDepartment of Chemistry and Center for Advanced Photonics Research, Temple University, Philadelphia, PA, 19122, USA. rjlevis@temple.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Charge state distributions are measured using mass spectrometry for both native and denatured cytochrome c and myoglobin after laser vaporization from the solution state into an electrospray (ES) plume consisting of a series of solution additives differing in gas-phase basicity. The charge distribution depends on both the pH of the protein solution prior to laser vaporization and the gas-phase basicity of the solution additive employed in the ES solvent. Cytochrome c (myoglobin) prepared in solutions with pH of 7.0, 2.6, and 2.3 resulted in the average charge state distribution (Z

Indexed as

Charge state distributionElectrospray ionizationFemtosecond laser vaporizationProtein conformation

Identifiers

PMID28063091

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.