Evidence map›Paper›PMID 28063000›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2017

Evaluation of loop-mediated isothermal amplification for the rapid identification of bacteria and resistance determinants in positive blood cultures.

J Rödel, J A Bohnert, S Stoll, L Wassill, B Edel, M Karrasch, B Löffler, W Pfister

Abstract readEvaluation Study
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In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Diagnostic methods and protocols for rapid determination of methicillin resistance in Staphylococcus aureus bloodstream infections: a comparative analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025
    Article
  3. Review
  4. Article
  5. Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2022
    Article
  6. The Evaluation of EazyplexAntibiotics (Basel, Switzerland) · 2022
    Article
  7. Article
  8. HumanMicroorganisms · 2020
    Review
  9. Rapid Detection of Methicillin-ResistantDiagnostics (Basel, Switzerland) · 2020
    Review
  10. Diagnostic accuracy and clinical impact of loop-mediated isothermal amplification for rapid detection of Staphylococcus aureus bacteremia: a retrospective observational study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020
    Observational
  11. Review
  12. Rapid culture-based identification of Shiga toxin-producing Escherichia coli and Shigella spp./Enteroinvasive E. coli using the eazyplex® EHEC complete assay.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020
    Article
  13. CelB is a suitable marker for rapid and specific identification of Klebsiella pneumoniae by the loop-mediated isothermal amplification (LAMP) assay.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019
    Article
  14. A 72-h intervention for improvement of the rate of optimal antibiotic therapy in patients with bloodstream infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

J RödelInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany. juergen.roedel@med.uni-jena.de.
J A BohnertInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany.
S StollInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany.
L WassillAmplexDiagnostics GmbH, Gars Bahnhof, Germany.
B EdelInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany.
M KarraschInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany.
B LöfflerInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany.
W PfisterInstitute of Medical Microbiology, Jena University Hospital, Jena, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The use of molecular assays to rapidly identify pathogens and resistance genes directly from positive blood cultures (BCs) contribute to shortening the time required for the diagnosis of bloodstream infections. In this work, loop-mediated isothermal amplification (LAMP) assays have been examined for their potential use in BC diagnosis. Three different assays were applied. The commercially available eazyplex® MRSA test detects Staphylococcus aureus, S. epidermidis, mecA, and mecC. Two in-house assays [Gram-positive (GP) and Gram-negative (GN)] have been developed for the detection of streptococci, enterococci, vanA, vanB, Pseudomonas spp., Enterobacteriaceae, and the bla

Indexed as

Blood CultureBacteremiaBacteriaDrug Resistance, BacterialGenotyping TechniquesHumansMicrobial Sensitivity TestsMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSensitivity and SpecificityTime Factors

Identifiers

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.