ArticleRNA (New York, N.Y.)2017
Survey of 800+ data sets from human tissue and body fluid reveals xenomiRs are likely artifacts.
Article in RNA (New York, N.Y.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed.
- Article
- An Apple a Day? The Hypothesis of Cross-Kingdom Gene Regulation by Plant miRNAs in Mammals and Its Controversies.International journal of molecular sciences · 2026Review
- The Conundrum of XenomiRs and Human Health.Advances in nutrition (Bethesda, Md.) · 2025Review
- Bioinformatics Analysis Reveals PPR Genes Modulation by Ahyp-miR0005 Under Abiotic Stress Across Diverse Plant Species.Plants (Basel, Switzerland) · 2025Article
- Plant-derived exosome-like nanoparticles for microRNA delivery in cancer treatment.Drug delivery and translational research · 2025Review
- MicroRNAs from edible plants reach the human gastrointestinal tract and may act as potential regulators of gene expression.Journal of physiology and biochemistry · 2024Article
- Species-specific microRNA discovery and target prediction in the soybean cyst nematode.Scientific reports · 2023Article
- miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages.Frontiers in nutrition · 2023Article
- Identifying the Potential of miRNAs inInternational journal of nanomedicine · 2023Article
- Exosome Carrier Effects; Resistance to Digestion in Phagolysosomes May Assist Transfers to Targeted Cells; II Transfers of miRNAs Are Better Analyzed via Systems Approach as They Do Not Fit Conventional Reductionist Stoichiometric Concepts.International journal of molecular sciences · 2022Review
- Milk exosomes in nutrition and drug delivery.American journal of physiology. Cell physiology · 2022Review
- Accurate quantification of 3'-terminal 2'-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR.Frontiers of medicine · 2022Article
- RNAi for Western Corn Rootworm Management: Lessons Learned, Challenges, and Future Directions.Insects · 2022Review
- Investigation of the transability of dietary small non-coding RNAs to animals.Frontiers in genetics · 2022Article
- The Challenges and Opportunities in the Development of MicroRNA Therapeutics: A Multidisciplinary Viewpoint.Cells · 2021Review
- Trans-kingdom RNAs and their fates in recipient cells: advances, utilization, and perspectives.Plant communications · 2021Review
- Large-scale identification of extracellular plant miRNAs in mammals implicates their dietary intake.PloS one · 2021Article
- Potential Mechanisms Linking Food-Derived MicroRNAs, Gut Microbiota and Intestinal Barrier Functions in the Context of Nutrition and Human Health.Frontiers in nutrition · 2021Review
- Circulating microRNA trafficking and regulation: computational principles and practice.Briefings in bioinformatics · 2020Review
- Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer.Oncotarget · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
miRNAs are small 22-nucleotide RNAs that can post-transcriptionally regulate gene expression. It has been proposed that dietary plant miRNAs can enter the human bloodstream and regulate host transcripts; however, these findings have been widely disputed. We here conduct the first comprehensive meta-study in the field, surveying the presence and abundances of cross-species miRNAs (xenomiRs) in 824 sequencing data sets from various human tissues and body fluids. We find that xenomiRs are commonly present in tissues (17%) and body fluids (69%); however, the abundances are low, comprising 0.001% of host human miRNA counts. Further, we do not detect a significant enrichment of xenomiRs in sequencing data originating from tissues and body fluids that are exposed to dietary intake (such as liver). Likewise, there is no significant depletion of xenomiRs in tissues and body fluids that are relatively separated from the main bloodstream (such as brain and cerebro-spinal fluids). Interestingly, the majority (81%) of body fluid xenomiRs stem from rodents, which are a rare human dietary contribution but common laboratory animals. Body fluid samples from the same studies tend to group together when clustered by xenomiR compositions, suggesting technical batch effects. Last, we performed carefully designed and controlled animal feeding studies, in which we detected no transfer of plant miRNAs into rat blood, or bovine milk sequences into piglet blood. In summary, our comprehensive computational and experimental results indicate that xenomiRs originate from technical artifacts rather than dietary intake.
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