ArticleMethods in enzymology2017
High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy.
Article in Methods in enzymology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Base-pair scale dynamics of a repair helicase on DNA lesions reveal varied damage-sensing mechanisms.bioRxiv : the preprint server for biology · 2026Article
- Article
- FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediate.Molecular cell · 2025Article
- Pleomorphic effects of three small-molecule inhibitors on transcription elongation bybioRxiv : the preprint server for biology · 2025Article
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Optical traps induce fluorophore photobleaching by two-photon excitation.Biophysical journal · 2023Article
- High-throughput force measurement of individual kinesin-1 motors during multi-motor transport.Nanoscale · 2022Article
- Assignment of structural transitions during mechanical unwrapping of nucleosomes and their disassembly products.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optical tweezer and TIRF microscopy for single molecule manipulation of RNA/DNA nanostructures including their rubbery property and single molecule counting.Biophysics reports · 2021Article
- Laser tweezers as a biophotonic tool to investigate the efficacy of living sickle red blood cells in response to optical deformation.Biophysical reviews · 2021Article
- DNA replication machinery: Insights fromComputational and structural biotechnology journal · 2021Review
- ALS/FTLD-Linked Mutations in FUS Glycine Residues Cause Accelerated Gelation and Reduced Interactions with Wild-Type FUS.Molecular cell · 2020Article
- Observation of processive telomerase catalysis using high-resolution optical tweezers.Nature chemical biology · 2020Article
- Article
- Regulation of Rep helicase unwinding by an auto-inhibitory subdomain.Nucleic acids research · 2019Article
- Bio-Molecular Applications of Recent Developments in Optical Tweezers.Biomolecules · 2019Review
- A force sensor that converts fluorescence signal into force measurement utilizing short looped DNA.Biosensors & bioelectronics · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We describe the design, construction, and application of an instrument combining dual-trap, high-resolution optical tweezers and a confocal microscope. This hybrid instrument allows nanomechanical manipulation and measurement simultaneously with single-molecule fluorescence detection. We present the general design principles that overcome the challenges of maximizing optical trap resolution while maintaining single-molecule fluorescence sensitivity, and provide details on the construction and alignment of the instrument. This powerful new tool is just beginning to be applied to biological problems. We present step-by-step instructions on an application of this technique that highlights the instrument's capabilities, detecting conformational dynamics in a nucleic acid-processing enzyme.
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Registered trials
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