ArticleBMC plant biology2017
Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- The old nourishes the new: 100 years of leaf senescence studies in Plant Physiology.Plant physiology · 2026Review
- Expression of a constitutively active nitrate reductase increases SARS-CoV-2 Spike protein production in Nicotiana benthamiana leaves that otherwise show traits of senescence.Plant biotechnology journal · 2026Article
- Activity-Based Profiling of Papain-like Cysteine Proteases During Late-Stage Leaf Senescence in Barley.Plants (Basel, Switzerland) · 2025Article
- Four guidelines to identify bona fide protease-substrate pairs.Journal of experimental botany · 2025Review
- Article
- Chlorine Modulates Photosynthetic Efficiency, Chlorophyll Fluorescence in Tomato Leaves, and Carbohydrate Allocation in Developing Fruits.International journal of molecular sciences · 2025Article
- Multi-scale phenotyping of senescence-related changes in roots of rapeseed in response to nitrate limitation.Journal of experimental botany · 2025Article
- Plant cathepsin B, a versatile protease.Frontiers in plant science · 2024Review
- Improving crop yield potential: Underlying biological processes and future prospects.Food and energy security · 2023Review
- Identification of Cognate Protease/Substrate Pairs by Use of Class-Specific Inhibitors.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Transient proteolysis reduction ofFrontiers in plant science · 2022Article
- Plant asparaginyl endopeptidases and their structural determinants of function.Biochemical Society transactions · 2021Review
- Autophagy and Nutrients Management in Plants.Cells · 2019Review
- Integrated Genome-Scale Analysis Identifies Novel Genes and Networks Underlying Senescence in Maize.The Plant cell · 2019Article
- Plant proteases during developmental programmed cell death.Journal of experimental botany · 2019Review
- The Role and Regulation of Autophagy and the Proteasome During Aging and Senescence in Plants.Genes · 2019Review
- Chloroplast Protein Degradation in Senescing Leaves: Proteases and Lytic Compartments.Frontiers in plant science · 2019Review
- Increases in activity of proteasome and papain-like cysteine protease in Arabidopsis autophagy mutants: back-up compensatory effect or cell-death promoting effect?Journal of experimental botany · 2018Article
- A New Role for SAG12 Cysteine Protease in Roots ofFrontiers in plant science · 2018Article
- Role of Papain-Like Cysteine Proteases in Plant Development.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPapain-like Cys Proteases (PLCPs) and Vacuolar Processing Enzymes (VPEs) are amongst the most highly expressed proteases during leaf senescence in Arabidopsis. Using activity-based protein profiling (ABPP), a method that enables detection of active enzymes within a complex sample using chemical probes, the activities of PLCPs and VPEs were investigated in individually darkened leaves of Arabidopsis, and their role in senescence was tested in null mutants.
resultsABPP and mass spectrometry revealed an increased activity of several PLCPs, particularly RD21A and AALP. By contrast, despite increased VPE transcript levels, active VPE decreased in individually darkened leaves. Eight protease knock-out lines and two protease over expressing lines were subjected to senescence phenotype analysis to determine the importance of individual protease activities to senescence. Unexpectedly, despite the absence of dominating PLCP activities in these plants, the rubisco and chlorophyll decline in individually darkened leaves and the onset of whole plant senescence were unaltered. However, a significant delay in progression of whole plant senescence was observed in aalp-1 and rd21A-1/aalp-1 mutants, visible in the reduced number of senescent leaves.
conclusionsMajor Cys protease activities are not essential for dark-induced and developmental senescence and only a knock out line lacking AALP shows a slight but significant delay in plant senescence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.