ArticleBMC genomics2017
The extracellular proteome of two Bifidobacterium species reveals different adaptation strategies to low iron conditions.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Breastfeeding Affects Concentration of Faecal Short Chain Fatty Acids During the First Year of Life: Results of the Systematic Review and Meta-Analysis.Frontiers in nutrition · 2022Pooled it
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- Differential impacts of hemin and free iron on amoxicillin susceptibility inFrontiers in microbiology · 2025Article
- The impact of iron and heme availability on the healthy human gut microbiome in vivo and in vitro.Cell chemical biology · 2023Article
- Comparative genomic analysis revealed genetic divergence between Bifidobacterium catenulatum subspecies present in infant versus adult guts.BMC microbiology · 2022Article
- Key bacterial taxa and metabolic pathways affecting gut short-chain fatty acid profiles in early life.The ISME journal · 2021Article
- Proteomic analysis and optimized production of Alkalihalobacillus patagoniensis PAT 05Bioprocess and biosystems engineering · 2021Article
- Bifidobacterium bifidum ATCC 15696 and Bifidobacterium breve 24b Metabolic Interaction Based on 2'-Applied and environmental microbiology · 2019Article
- Positive Selection inFrontiers in microbiology · 2019Article
- Clustering of Pan- and Core-genome of Lactobacillus provides Novel Evolutionary Insights for Differentiation.BMC genomics · 2018Article
- Genome-Wide Search for Genes Required for Bifidobacterial Growth under Iron-Limitation.Frontiers in microbiology · 2017Article
- Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBifidobacteria are among the first anaerobic bacteria colonizing the gut. Bifidobacteria require iron for growth and their iron-sequestration mechanisms are important for their fitness and possibly inhibit enteropathogens. Here we used combined genomic and proteomic analyses to characterize adaptations to low iron conditions of B. kashiwanohense PV20-2 and B. pseudolongum PV8-2, 2 strains isolated from the feces of iron-deficient African infants and selected for their high iron-sequestering ability.
resultsAnalyses of the genome contents revealed evolutionary adaptation to low iron conditions. A ferric and a ferrous iron operon encoding binding proteins and transporters were found in both strains. Remarkably, the ferric iron operon of B. pseudolongum PV8-2 is not found in other B. pseudolongum strains and likely acquired via horizontal gene transfer. The genome B. kashiwanohense PV20-2 harbors a unique region encoding genes putatively involved in siderophore production. Additionally, the secretomes of the two strains grown under low-iron conditions were analyzed using a combined genomic-proteomic approach. A ferric iron transporter was found in the secretome of B. pseudolongum PV8-2, while ferrous binding proteins were detected in the secretome of B. kashiwanohense PV20-2, suggesting different strategies to take up iron in the strains. In addition, proteins such as elongation factors, a glyceraldehyde-3-phosphate dehydrogenase, and the stress proteins GroEL and DnaK were identified in both secretomes. These proteins have been previously associated with adhesion of lactobacilli to epithelial cells.
conclusionAnalyses of the genome and secretome of B. kashiwanohense PV20-2 and B. pseudolongum PV8-2 revealed different adaptations to low iron conditions and identified extracellular proteins for iron transport. The identified extracellular proteins might be involved in competition for iron in the gastrointestinal tract.
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