ArticleBMC genomics2017
Shotgun proteomics of the barley seed proteome.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- The Influence of Barley Proteome on Hop Bitter Acid Yield during Brewing.Journal of agricultural and food chemistry · 2024Article
- Snapshot of four mature quinoa (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Proteomic Analysis of Pecan (Foods (Basel, Switzerland) · 2023Article
- Comparative Proteomics of Potato Cultivars with a Variable Dormancy Period.Molecules (Basel, Switzerland) · 2022Article
- Paleoproteomics.Chemical reviews · 2022Review
- Seed Development and Protein Accumulation Patterns in Faba Bean (Journal of agricultural and food chemistry · 2022Article
- Integrative metabolomic and transcriptomic analyses reveal the mechanisms of Tibetan hulless barley grain coloration.Frontiers in plant science · 2022Article
- Application of Mass Spectrometry-Based Proteomics to Barley Research.Journal of agricultural and food chemistry · 2021Review
- The post-translational modification landscape of commercial beers.Scientific reports · 2021Article
- Barley Seeds miRNome Stability during Long-Term Storage and Aging.International journal of molecular sciences · 2021Article
- Benchtop micro-mashing: high-throughput, robust, experimental beer brewing.Scientific reports · 2021Article
- Shotgun proteomics of Brassica rapa seed proteins identifies vicilin as a major seed storage protein in the mature seed.PloS one · 2021Article
- Comparative de novo transcriptome analysis of barley varieties with different malting qualities.Functional & integrative genomics · 2020Article
- TGFam-Finder: a novel solution for target-gene family annotation in plants.The New phytologist · 2020Article
- PANOMICS meets germplasm.Plant biotechnology journal · 2020Review
- Analysis of the Barley Malt Rootlet Proteome.International journal of molecular sciences · 2019Article
- Subcellular Proteomics: Application to Elucidation of Flooding-Response Mechanisms in Soybean.Proteomes · 2018Review
- Microscopic and Proteomic Analysis of Dissected Developing Barley Endosperm Layers Reveals the Starchy Endosperm as Prominent Storage Tissue for ER-Derived Hordeins Alongside the Accumulation of Barley Protein Disulfide Isomerase (HvPDIL1-1).Frontiers in plant science · 2018Article
- Using RT-qPCR, Proteomics, and Microscopy to Unravel the Spatio-Temporal Expression and Subcellular Localization of Hordoindolines Across Development in Barley Endosperm.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBarley seed proteins are of prime importance to the brewing industry, human and animal nutrition and in plant breeding for cultivar identification. To obtain comprehensive proteomic data from seeds, total protein from a two-rowed (Conrad) and a six-rowed (Lacey) barley cultivar were precipitated in acetone, digested in-solution, and the resulting peptides were analyzed by nano-liquid chromatography coupled with tandem mass spectrometry.
resultsThe raw mass spectra data searched against Uniprot's Barley database using in-house Mascot search engine identified 1168 unique proteins. Gene Ontology (GO) analysis indicated that the majority of the seed proteins were cytosolic, with catalytic activity and associated with carbohydrate metabolism. Spectral counting analysis showed that there are 20 differentially abundant seed proteins between the two-rowed Conrad and six-rowed Lacey cultivars.
conclusionThis study paves the way for the use of a top-down gel-free proteomics strategy in barley for investigating more complex traits such as malting quality. Differential abundance of hordoindoline proteins impact the seed hardness trait of barley cultivars.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.