ArticlePloS one2017
Clock Genes Regulate the Circadian Expression of Piezo1, TRPV4, Connexin26, and VNUT in an Ex Vivo Mouse Bladder Mucosa.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Piezo channels in physical field therapy: From mechanotransduction mechanisms to bioelectromagnetic modulation and biomedical applications.Mechanobiology in medicine · 2026Review
- Elucidating Characteristics of Co-Existent Overactive and Underactive Bladder in Participants of the 2023 Japan Community Health Survey (JaCS 2023) With Nocturia.Neurourology and urodynamics · 2026Article
- Biology and Time Course of Obstruction-Induced Detrusor Underactivity Causing Postvoid Residual Urine.International neurourology journal · 2026Article
- Tuina ameliorates sleep disturbances in PCPA-treated rats through Piezo1-mediated calcium signaling.Frontiers in pharmacology · 2026Article
- The Mechanosensation-Metabolism-Inflammation Axis: The Central Role of Piezo1 and TRPV4 in Hypertension-Related Atherosclerosis.International journal of general medicine · 2026Review
- Diurnal regulation of urinary behavior and gene expression in aged mice.The journals of gerontology. Series A, Biological sciences and medical sciences · 2025Article
- Biological Fluid Flows: Signaling Mediums for Circadian Timing.Journal of biological rhythms · 2025Review
- The role of TRPV4 in acute sleep deprivation-induced memory impairment: Mechanisms of calcium dysregulation and synaptic plasticity disruption.Cell insight · 2025Article
- Disruption of circadian rhythm as a potential pathogenesis of nocturia.Nature reviews. Urology · 2025Review
- Mechanisms of oxidative stress in interstitial cystitis/bladder pain syndrome.Nature reviews. Urology · 2024Review
- The physiological role of TRP channels in sleep and circadian rhythm.Journal of cellular and molecular medicine · 2024Review
- Glucocorticoids coordinate the bladder peripheral clock and diurnal micturition pattern in mice.Communications biology · 2023Article
- The Expression and Function of Piezo Channels in Bladder.Bladder (San Francisco, Calif.) · 2023Review
- UHRF1-induced connexin26 methylation is involved in hearing damage triggered by intermittent hypoxia in neonatal rats.Open medicine (Warsaw, Poland) · 2023Article
- Piezo channels in the urinary system.Experimental & molecular medicine · 2022Review
- Timing is everything: impact of development, ageing and circadian rhythm on macrophage functions in urinary tract infections.Mucosal immunology · 2022Review
- Effects of fatty acid metabolites on nocturia.Scientific reports · 2022Article
- A trio of biological rhythms and their relevance in rhythmic mechanical stimulation of cell cultures.Frontiers in psychology · 2022Article
- Functional roles for PIEZO1 and PIEZO2 in urothelial mechanotransduction and lower urinary tract interoception.JCI insight · 2021Article
- Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesClockΔ19/Δ19 mice is an experimental model mouse for nocturia (NOC). Using the bladder mucosa obtained from ClockΔ19/Δ19 mice, we investigated the gene expression rhythms of mechanosensory cation channels such as transient receptor potential cation channel subfamily V member 4 (TRPV4) and Piezo1, and main ATP release pathways including vesicular nucleotide transporter (VNUT) and Connexin26(Cx26), in addition to clock genes. MATERIALS AND
methodsEight- to twelve-week-old male C57BL/6 mice (WT) and age- and sex-matched C57BL/6 ClockΔ19/Δ19 mice, which were bred under 12-h light/dark conditions for 2 weeks, were used. Gene expression rhythms and transcriptional regulation mechanisms in clock genes, mechanosensor, Cx26 and VNUT were measured in the mouse bladder mucosa, collected every 4 hours from WT and ClockΔ19/Δ19 mice using quantitative RT-PCR, a Western blot analysis, and ChIP assays.
resultsWT mice showed circadian rhythms in clock genes as well as mechanosensor, Cx26 and VNUT. Their expression was low during the sleep phase. The results of ChIP assays showed Clock protein binding to the promotor regions and the transcriptional regulation of mechanosensor, Cx26 and VNUT. In contrast, all of these circadian expressions were disrupted in ClockΔ19/Δ19 mice. The gene expression of mechanosensor, Cx26 and VNUT was maintained at a higher level in spite of the sleep phase.
conclusionsMechanosensor, Cx26 and VNUT expressed with circadian rhythm in the mouse bladder mucosa. The disruption of circadian rhythms in these genes, induced by the abnormalities in clock genes, may be factors contributing to NOC because of hypersensitivity to bladder wall extension.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.