ArticlePloS one2017
Developing and Testing a Bayesian Analysis of Fluorescence Lifetime Measurements.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Multi-parameter fluorescence lifetime imaging for high-noise neuroscience applications.Biomedical optics express · 2026Article
- Fluorescence Lifetime Microscopy Methods for Studying Dynamics of Fluorescent Proteins In Vivo and In Vitro.Advances in experimental medicine and biology · 2026Review
- Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations.ACS photonics · 2023Article
- Article
- Optimal inference of molecular interaction dynamics in FRET microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Fluorescence lifetime: Beating the IRF and interpulse window.Biophysical journal · 2023Article
- High Resolution Fluorescence Lifetime Maps from Minimal Photon Counts.ACS photonics · 2022Article
- Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Lifetime-based analysis of binary fluorophores mixtures in the low photon count limit.iScience · 2022Article
- Complex wavelet filter improves FLIM phasors for photon starved imaging experiments.Biomedical optics express · 2021Article
- Blind deconvolution estimation by multi-exponential models and alternated least squares approximations: Free-form and sparse approach.PloS one · 2021Article
- Direct Photon-by-Photon Analysis of Time-Resolved Pulsed Excitation Data using Bayesian Nonparametrics.Cell reports. Physical science · 2020Article
- Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.Journal of biomedical optics · 2020Review
- Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue.Theranostics · 2020Article
- Nonparametric empirical Bayesian framework for fluorescence-lifetime imaging microscopy.Biomedical optics express · 2019Article
- Article
- Bridging length scales to measure polymer assembly.Molecular biology of the cell · 2017Article
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Authors and funding
4 authors.
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Abstract
FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM), which relies on the reduction of fluorescence lifetime when a fluorophore is undergoing FRET. FLIM measurements take the form of histograms of photon arrival times, containing contributions from a mixed population of fluorophores both undergoing and not undergoing FRET, with the measured distribution being a mixture of exponentials of different lifetimes. Here, we present an analysis method based on Bayesian inference that rigorously takes into account several experimental complications. We test the precision and accuracy of our analysis on controlled experimental data and verify that we can faithfully extract model parameters, both in the low-photon and low-fraction regimes.
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