ArticlePLoS neglected tropical diseases2017
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 83 citations in OpenAlex.
- Metagenomic analysis of bacterial and viral communities of Aedes aegypti and Aedes albopictus.Journal, genetic engineering & biotechnology · 2026Article
- Dietary Lipids and Their Metabolism in the Midgut.Advances in experimental medicine and biology · 2026Review
- Intraspecific microbiome dynamics across the life cycle of the milkweed bugMicrobial genomics · 2026Article
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
- Article
- Selected gut bacteria promote mosquito larval growth through the regulation of ionic microenvironment and protease expression.iScience · 2025Article
- Bacteria associated with the cereal leaf beetle act as the insect's allies in adapting to protease inhibitors, but impair its development in laboratory condition.Scientific reports · 2025Article
- Mosquito host background impacts microbiome-Zika virus interactions in field- and laboratory-reared Aedes aegypti.Animal microbiome · 2025Article
- Exploring the role of microbiota in mediating sexually dimorphic infection outcomes in mealworm beetles.Biology letters · 2025Article
- Variation in diet concentration and bacterial inoculum size in larval habitats shapes the performance of the Asian tiger mosquito, Aedes albopictus.Microbiome · 2025Article
- Microbial composition and function are nested and shaped by food web topologies.ISME communications · 2025Article
- Symbiotic Bacteria: Wolbachia, Midgut Microbiota in Mosquitoes and Their Importance for Vector Prevention Strategies.Microbial ecology · 2024Review
- Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes.Environmental microbiology · 2024Article
- Increased environmental microbial diversity reduces the disease risk of a mosquitocidal pathogen.mBio · 2024Article
- The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence.Communications biology · 2023Article
- Microbiota in disease-transmitting vectors.Nature reviews. Microbiology · 2023Review
- Absence of microbiome triggers extensive changes in the transcriptional profile of Hermetia illucens during larval ontology.Scientific reports · 2023Article
- The buzz in the field: the interaction between viruses, mosquitoes, and metabolism.Frontiers in cellular and infection microbiology · 2023Review
- Recovery of metagenomic data from theWellcome open research · 2023Article
- Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes.Microbiome · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. We previously reported that Aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only Escherichia coli develop very similarly into adults, whereas axenic larvae never molt and die as first instars. In this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during the first instar. Results showed that conventional and gnotobiotic larvae exhibited no differences in growth, timing of molting, or number of bacteria in their digestive tract. Axenic larvae in contrast grew minimally and never achieved the critical size associated with molting by conventional and gnotobiotic larvae. In the second part of the study we compared patterns of gene expression in conventional, gnotobiotic and axenic larvae by conducting an RNAseq analysis of gut and nongut tissues (carcass) at 22 h post-hatching. Approximately 12% of Ae. aegypti transcripts were differentially expressed in axenic versus conventional or gnotobiotic larvae. However, this profile consisted primarily of transcripts in seven categories that included the down-regulation of select peptidases in the gut and up-regulation of several genes in the gut and carcass with roles in amino acid transport, hormonal signaling, and metabolism. Overall, our results indicate that axenic larvae exhibit alterations in gene expression consistent with defects in acquisition and assimilation of nutrients required for growth.
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