ArticleCurrent protocols in molecular biology2017
Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans.
Article in Current protocols in molecular biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
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- CMTR-1 RNA methyltransferase mutations activate widespread expression of a dopaminergic neuron-specific mitochondrial complex I gene.Current biology : CB · 2024Article
- Hypoxia-inducible factor induces cysteine dioxygenase and promotes cysteine homeostasis ineLife · 2024Article
- Hypoxia and intra-complex genetic suppressors rescue complex I mutants by a shared mechanism.Cell · 2024Article
- Hypoxia-inducible factor induces cysteine dioxygenase and promotes cysteine homeostasis inbioRxiv : the preprint server for biology · 2023Article
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- C. elegans: A biosensor for host-microbe interactions.Lab animal · 2021Review
- Drug discovery: Insights from the invertebrate Caenorhabditis elegans.Pharmacology research & perspectives · 2021Review
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- The dual benefit of a dominant mutation in Arabidopsis IRON DEFICIENCY TOLERANT1 for iron biofortification and heavy metal phytoremediation.Plant biotechnology journal · 2020Article
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- Molybdenum cofactor transfer from bacteria to nematode mediates sulfite detoxification.Nature chemical biology · 2019Article
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- Whole Genome Next-Generation Sequencing Mutation Identification in Pseudomonas aeruginosa.Current protocols in molecular biology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Forward genetic analysis using chemical mutagenesis in model organisms is a powerful tool for investigation of molecular mechanisms in biological systems. In the nematode, Caenorhabditis elegans, mutagenesis screens using ethyl methanesulfonate (EMS) have led to important insights into genetic control of animal development and physiology. A major bottleneck to this approach is identification of the causative mutation underlying a phenotype of interest. In the past, this has required time-consuming genetic mapping experiments. More recently, next-generation sequencing technologies have allowed development of new methods for rapid mapping and identification of EMS-induced lesions. In this unit we describe a protocol to map and identify EMS-induced mutations in C. elegans. © 2017 by John Wiley & Sons, Inc.
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