ArticleThe ISME journal2017
Single-cell transcriptomics of small microbial eukaryotes: limitations and potential.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Marine microbiomes and their expanding role in biotechnological potential: a systematic review.Archives of microbiology · 2026Pooled it
- A contamination-reduced genome of Plasmodiophora brassicae with comprehensive functional annotation.BMC microbiology · 2026Article
- Phylogeny and species delimitation of ciliates in the genus Spirostomum (class Heterotrichea) using single-cell transcriptomes.BMC ecology and evolution · 2025Article
- Proportional relationship between transcript concentrations and carbon biomass for open ocean plankton groups.The ISME journal · 2025Article
- Cellular heterogeneity in metabolism and associated microbiome of a non-model phytoflagellate.The ISME journal · 2025Article
- Phylogeny and species delimitation of ciliates in the genusbioRxiv : the preprint server for biology · 2024Article
- Towards high-throughput parallel imaging and single-cell transcriptomics of microbial eukaryotic plankton.PloS one · 2024Article
- Group-specific functional patterns of mitochondrion-related organelles shed light on their multiple transitions from mitochondria in ciliated protists.Marine life science & technology · 2022Article
- One Cell at a Time: Advances in Single-Cell Methods and Instrumentation for Discovery in Aquatic Microbiology.Frontiers in microbiology · 2022Article
- Division of functional roles for termite gut protists revealed by single-cell transcriptomes.The ISME journal · 2020Article
- An efficient single-cell transcriptomics workflow for microbial eukaryotes benchmarked on Giardia intestinalis cells.BMC genomics · 2020Article
- Article
- Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- A single-cell genome perspective on studying intracellular associations in unicellular eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Combining morphology, behaviour and genomics to understand the evolution and ecology of microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- A practical implementation of large transcriptomic data analysis to resolve cryptic species diversity problems in microbial eukaryotes.BMC evolutionary biology · 2018Article
- Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions.Microbiology and molecular biology reviews : MMBR · 2018Review
- Single Cell Transcriptomics, Mega-Phylogeny, and the Genetic Basis of Morphological Innovations in Rhizaria.Molecular biology and evolution · 2017Article
- Quest of Soil Protists in a New Era.Microbes and environments · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell transcriptomics is an emerging research tool that has huge untapped potential in the study of microbial eukaryotes. Its application has been tested in microbial eukaryotes 50 μm or larger, and it generated transcriptomes similar to those obtained from culture-based RNA-seq. However, microbial eukaryotes have a wide range of sizes and can be as small as 1 μm. Single-cell RNA-seq was tested in two smaller protists (8 and 15 μm). Transcript recovery rate was much lower and randomness in observed gene expression levels was much higher in single-cell transcriptomes than those derived from bulk cultures of cells. We found that the reason of such observation is that the smaller organisms had much lower mRNA copy numbers. We discuss the application of single-cell RNA-seq in studying smaller microbial eukaryotes in the context of these limitations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.