ArticleJournal of visualized experiments : JoVE2016
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- Dynamin inhibitor dynasore modulates longitudinal bone growth in a hormetic manner.BMC biology · 2026Article
- Pannexin 3-mediated adenosine triphosphate release from pre-osteoblasts promotes osteoblast differentiation through P2X4 receptor activation.Frontiers in physiology · 2026Article
- Exploring the impact of mechanical stimuli on growth plate morphology and trabecular adaptation: A finite element approach.Journal of orthopaedics · 2025Article
- Fibroblast Growth Factor 19 Disrupts Cartilage Development Via the FGFR4/β-catenin Axis.International journal of biological sciences · 2025Article
- Type-H endothelial cell protein Clec14a orchestrates osteoblast activity during trabecular bone formation and patterning.Communications biology · 2024Article
- Growth and mineralization of fetal mouse long bones under microgravity and daily 1 g gravity exposure.NPJ microgravity · 2024Article
- Humanin Treatment Protects Against Venetoclax-Induced Bone Growth Retardation inJournal of the Endocrine Society · 2024Article
- Article
- Restraint upon Embryonic MetatarsalInternational journal of molecular sciences · 2021Article
- Role of extracellular vesicles from adipose tissue- and bone marrow-mesenchymal stromal cells in endothelial proliferation and chondrogenesis.Stem cells translational medicine · 2021Article
- A Comparison of Osteoblast and Osteoclast In Vitro Co-Culture Models and Their Translation for Preclinical Drug Testing Applications.International journal of molecular sciences · 2020Review
- A comparison of the bone and growth phenotype ofDisease models & mechanisms · 2020Article
- Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.Cellular and molecular life sciences : CMLS · 2019Article
- Postnatal ex vivo rat model for longitudinal bone growth investigations.Animal models and experimental medicine · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth. As such, it is imperative that we further our understanding of the underpinning molecular mechanisms involved in such disorders so as to provide advances towards human and animal patient benefit. The mouse metatarsal organ explant culture is a highly physiological ex vivo model for studying endochondral ossification and bone growth as the growth rate of the bones in culture mimic that observed in vivo. Uniquely, the metatarsal organ culture allows the examination of chondrocytes in different phases of chondrogenesis and maintains cell-cell and cell-matrix interactions, therefore providing conditions closer to the in vivo situation than cells in monolayer or 3D culture. This protocol describes in detail the intricate dissection of embryonic metatarsals from the hind limb of E15 murine embryos and the subsequent analyses that can be performed in order to examine endochondral ossification and longitudinal bone growth.
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