ArticleJournal of visualized experiments : JoVE2016
Mass Histology to Quantify Neurodegeneration in Drosophila.
Article in Journal of visualized experiments : JoVE, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Article
- The cyclic nucleotide binding sites of Swiss-Cheese, thebioRxiv : the preprint server for biology · 2025Article
- VacQuant: a tool to quantify neurodegeneration and associated vacuolation in brain tissue.Fly · 2025Article
- Reduced expression ofJournal of Parkinson's disease · 2025Article
- An integrative systems-biology approach defines mechanisms of Alzheimer's disease neurodegeneration.Nature communications · 2025Article
- Adverse impact of female reproductive signaling on age-dependent neurodegeneration after mild head trauma ineLife · 2024Article
- Heterochromatic silencing of immune-related genes in glia is required for BBB integrity and normal lifespan in drosophila.Aging cell · 2023Article
- Article
- Parkinsonism mutations in DNAJC6 cause lipid defects and neurodegeneration that are rescued by Synj1.NPJ Parkinson's disease · 2023Article
- String/Cdc25 phosphatase is a suppressor of Tau-associated neurodegeneration.Disease models & mechanisms · 2023Article
- Review
- An optimized temporally controlled Gal4 system inFrontiers in neuroscience · 2023Article
- Review
- Eosin whole-brain mount staining to analyze neurodegeneration in a fly model of Alzheimer's disease.STAR protocols · 2022Article
- A protocol to detect neurodegeneration inSTAR protocols · 2021Article
- dCubilin- or dAMN-mediated protein reabsorption in Drosophila nephrocytes modulates longevity.Disease models & mechanisms · 2021Article
- Article
- Disease-Associated Mutant Tau Prevents Circadian Changes in the Cytoskeleton of Central Pacemaker Neurons.Frontiers in neuroscience · 2020Article
- Micro-computed tomography as a platform for exploringDevelopment (Cambridge, England) · 2019Article
- ER responses play a key role in Swiss-Cheese/Neuropathy Target Esterase-associated neurodegeneration.Neurobiology of disease · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Progressive neurodegenerative diseases like Alzheimer's disease (AD) or Parkinson's disease (PD) are an increasing threat to human health worldwide. Although mammalian models have provided important insights into the underlying mechanisms of pathogenicity, the complexity of mammalian systems together with their high costs are limiting their use. Therefore, the simple but well-established Drosophila model-system provides an alternative for investigating the molecular pathways that are affected in these diseases. Besides behavioral deficits, neurodegenerative diseases are characterized by histological phenotypes such as neuronal death and axonopathy. To quantify neuronal degeneration and to determine how it is affected by genetic and environmental factors, we use a histological approach that is based on measuring the vacuoles in adult fly brains. To minimize the effects of systematic error and to directly compare sections from control and experimental flies in one preparation, we use the 'collar' method for paraffin sections. Neurodegeneration is then assessed by measuring the size and/or number of vacuoles that have developed in the fly brain. This can either be done by focusing on a specific region of interest or by analyzing the entire brain by obtaining serial sections that span the complete head. Therefore, this method allows one to measure not only severe degeneration but also relatively mild phenotypes that are only detectable in a few sections, as occurs during normal aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.