ArticleScientific reports2017
Comparison of sputum microbiome of legionellosis-associated patients and other pneumonia patients: indications for polybacterial infections.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- Therapeutical strategies in cavitary legionnaires' disease, two cases from the field and a systematic review.Annals of clinical microbiology and antimicrobials · 2023Pooled it
- Antibiotic Resistance ofInternational journal of environmental research and public health · 2020Pooled it
- Sputum lautropia is decreased in rheumatoid arthritis-associated pulmonary fibrosis.Arthritis research & therapy · 2026Article
- A Pilot Study ofDiagnostics (Basel, Switzerland) · 2026Article
- Metagenomic next-generation sequencing reveals airway microbial dysbiosis and functional alterations inFrontiers in cellular and infection microbiology · 2026Article
- Comparative genome analysis investigation of nosocomial and community-acquired cases of Legionnaires' disease caused by ST2858 and ST378.Microbiology spectrum · 2025Article
- Bacterial translocation and gut microbiome imbalance in an experimental infection model of legionellosis in guinea pigs.BMC microbiology · 2025Article
- Case report: The value of early application of mNGS technology in the diagnosis and treatment of severe Legionnaires' disease: reports of two cases with different outcomes.Frontiers in medicine · 2025Article
- Legionella Pneumonia Undetected by Repeated Urinary Antigen Testing With Ribotest® Legionella.Cureus · 2024Article
- Article
- Characteristics of the pulmonary microbiota in patients with mild and severe pulmonary infection.Frontiers in cellular and infection microbiology · 2023Article
- Characteristics of the lung microbiota in lower respiratory tract infections with and without history of pneumonia.Bioengineered · 2021Article
- Temporal dynamics of the plasma microbiome in recipients at early post-liver transplantation: a retrospective study.BMC microbiology · 2021Article
- A Comparison of Blood Pathogen Detection Among Droplet Digital PCR, Metagenomic Next-Generation Sequencing, and Blood Culture in Critically Ill Patients With Suspected Bloodstream Infections.Frontiers in microbiology · 2021Article
- Biogeography and Environmental Drivers ofPathogens (Basel, Switzerland) · 2020Article
- Article
- Next-generation sequencing specifies Angiostrongylus eosinophilic meningoencephalitis in infants: Two case reports.Medicine · 2019Article
- Antimicrobial agent susceptibilities of Legionella pneumophila MLVA-8 genotypes.Scientific reports · 2019Article
- A Haemophilus sp. dominates the microbiota of sputum from UK adults with non-severe community acquired pneumonia and chronic lung disease.Scientific reports · 2019Article
- Comprehensive detection of pathogens in immunocompromised children with bloodstream infections by next-generation sequencing.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria of the genus Legionella cause water-based infections resulting in severe pneumonia. Here we analyze and compare the bacterial microbiome of sputum samples from pneumonia patients in relation to the presence and abundance of the genus Legionella. The prevalence of Legionella species was determined by culture, PCR, and Next Generation Sequencing (NGS). Nine sputum samples out of the 133 analyzed were PCR-positive using Legionella genus-specific primers. Only one sample was positive by culture. Illumina MiSeq 16S rRNA gene sequencing analyses of Legionella-positive and Legionella-negative sputum samples, confirmed that indeed, Legionella was present in the PCR-positive sputum samples. This approach allowed the identification of the sputum microbiome at the genus level, and for Legionella genus at the species and sub-species level. 42% of the sputum samples were dominated by Streptococcus. Legionella was never the dominating genus and was always accompanied by other respiratory pathogens. Interestingly, sputum samples that were Legionella positive were inhabited by aquatic bacteria that have been observed in an association with amoeba, indicating that amoeba might have transferred Legionella from the drinking water together with its microbiome. This is the first study that demonstrates the sputum major bacterial commensals and pathogens profiles with regard to Legionella presence.
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Registered trials
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