Evidence map›Paper›PMID 28058692›Full record

ArticleDoklady. Biochemistry and biophysics2016

Comparative characteristics of two anion-channel rhodopsins and prospects of their use in optogenetics.

D A Dolgikh, A Yu Malyshev, M V Roshchin, G R Smirnova, O V Nekrasova, L E Petrovskaya, T B Feldman, P M Balaban, M P Kirpichnikov, M A Ostrovsky

Abstract readComparative Study
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In one paragraph

Article in Doklady. Biochemistry and biophysics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

D A DolgikhPirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, 117997, Russia.
A Yu MalyshevInstitute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, 11748, Russia.
M V RoshchinInstitute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, 11748, Russia.
G R SmirnovaInstitute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, 11748, Russia.
O V NekrasovaBiological Faculty, Moscow State University, Moscow, 119991, Russia.
L E PetrovskayaPirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, 117997, Russia.
T B FeldmanBiological Faculty, Moscow State University, Moscow, 119991, Russia.
P M BalabanInstitute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, 11748, Russia.
M P KirpichnikovBiological Faculty, Moscow State University, Moscow, 119991, Russia.
M A OstrovskyBiological Faculty, Moscow State University, Moscow, 119991, Russia. ostrovsky3535@mail.ru.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Anion-selective opsins slow ChloC and ACR2 were expressed in rat brain cortical neurons by electroporation in utero. It is shown that the light-activated channel ACR2 has pronounced advantages in terms of both the inactivation kinetics and the neuron inhibition intensity, which is associated with a more negative value of the light-activated current reversal potential compared to the slow ChloC channel. The identified properties of opsin ACR2 indicate that it can be used for strictly controlled suppression of neuronal activity in optogenetic experiments, including the expression in the retinal ganglionic cells for reconstituting the OFF-component of their receptive field, which is essential for optogenetic prosthetics of degenerative retina.

Indexed as

OptogeneticsAnimalsCerebral CortexElectroporationLightMembrane PotentialsNeuronsPatch-Clamp TechniquesRatsRhodopsinTissue Culture TechniquesVoltage-Dependent Anion Channel 2RhodopsinVoltage-Dependent Anion Channel 2

Identifiers

PMID28058692

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.