Evidence map›Paper›PMID 28058688›Full record

ArticleDoklady. Biochemistry and biophysics2016

Targeted mutagenesis of Drosophila RNaseZ gene by homologous recombination.

O V Andreenkov, E I Volkova, S A Demakov, X Xie, E B Dubrovsky, I F Zhimulev

Abstract read
PubMed Publisher
In one paragraph

Article in Doklady. Biochemistry and biophysics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

O V AndreenkovInstitute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia.
E I VolkovaInstitute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia.
S A DemakovInstitute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia. demakov@mcb.nsc.ru.
X XieFordham University, New York, USA.
E B DubrovskyFordham University, New York, USA.
I F ZhimulevInstitute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

For the first time we used a homologous recombination method to obtain complete and precise deletion of Drosophila dRNaseZ gene. In the founder line of flies in which the RNaseZ sequence was replaced by attP site, the full-length sequence of the gene was reintegrated, and its functionality was shown. This approach will allow us to generate further gene mutations in different domains of dRNaseZ protein and discover a broad spectrum and uncover functions outside of tRNA processing.

Indexed as

Genetic TechniquesHomologous RecombinationSequence DeletionAnimalsAnimals, Genetically ModifiedAttachment Sites, MicrobiologicalBlotting, NorthernBlotting, WesternCentral Nervous SystemChromosomes, Artificial, BacterialDrosophilaDrosophila ProteinsEndoribonucleasesFemaleGonadsImaginal DiscsDrosophila ProteinsEndoribonucleasesMutant Chimeric ProteinsRNARNA, MitochondrialRNaseZ protein, DrosophilaRNA, Transfer

Identifiers

PMID28058688

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.