ArticleACS omega2016
Quantification of Small Molecule-Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore.
Article in ACS omega, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 43 citations in OpenAlex.
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- Quantification of Binding of Small Molecules to Native Proteins Overexpressed in Living Cells.Journal of the American Chemical Society · 2024Article
- Autoinducer-fluorophore conjugates enable FRET in LuxR proteins in vitro and in cells.Nature chemical biology · 2022Article
- Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans.RSC advances · 2021Article
- An intrinsic FRET sensor of protein-ligand interactions.Organic & biomolecular chemistry · 2020Article
- Sensitive Label-Free Thermal Stability Assay for Protein Denaturation and Protein-Ligand Interaction Studies.Analytical chemistry · 2020Article
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- Antibody-Drug Conjugate that Exhibits Synergistic Cytotoxicity with an Endosome-Disruptive Peptide.ACS omega · 2019Article
- Convenient Synthesis of Functionalized Cyclopropa[Molecules (Basel, Switzerland) · 2018Article
- Semisynthesis and Biological Evaluation of Platensimycin Analogues with Varying Aminobenzoic Acids.ChemistrySelect · 2018Article
- Targeting Fluorescent Sensors to Endoplasmic Reticulum Membranes Enables Detection of Peroxynitrite During Cellular Phagocytosis.ACS chemical biology · 2018Article
- Synthesis of a Fluorescent Analogue of Paclitaxel That Selectively Binds Microtubules and Sensitively Detects Efflux by P-Glycoprotein.Angewandte Chemie (International ed. in English) · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of systems. To improve access to PB, we developed a scalable multigram synthesis of this fluorophore, starting with inexpensive 2,3,4,5-tetrafluorobenzoic acid. This route was used to synthesize fluorescent derivatives of biotin, as well as lower affinity thiobiotin, iminobiotin, and imidazolidinethione analogues that bind the protein streptavidin. Compared with previously published FRET acceptors for tryptophan, PB proved to be superior in both sensitivity and efficiency. These unique properties of PB enabled direct quantification of dissociation constants (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.