Evidence map›Paper›PMID 28057905›Full record

ArticleJournal of veterinary science2017

Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus.

Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang

Open access · goldAbstract read
In one paragraph

Article in Journal of veterinary science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.6field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Huawei LiCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Jifei YangHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Dengke BaoHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Jie HouHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Yubao ZhiHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Yanyan YangHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Pengchao JiCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Enmin ZhouCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Songlin QiaoHenan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Gaiping ZhangCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Henan Academy of Agricultural Sciences · CNNorthwest A&F University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with standard diagnostic tests, enzyme-linked immunosorbent assays and immunoperoxidase monolayer assay, was also performed. The immunochromatographic test strip was shown to be of high specificity and sensitivity. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. It is suggested that the immunochromatographic test strip can be used to quickly and accurately detect PRRSV antibody and to be suitable for diagnostic purposes in the field.

Indexed as

Reagent StripsAnimalsAntibodies, ViralBlotting, WesternChromatography, AffinityEnzyme-Linked Immunosorbent AssayPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusSwineAntibodies, ViralReagent Stripsenzyme-linked immunosorbent assayimmunochromatographyporcine reproductive and respiratory syndrome

Identifiers

PMID28057905
PMCPMC5639083
OpenAlexW2761881066

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.